A recombinant fragment of human surfactant protein D lacking the short collagen-like stalk fails to correct morphological alterations in lungs of SP-D deficient mice
Knudsen, Lars, Wucherpfennig, Katharina, Mackay, Rose-Marie, Townsend, Paul, Muhlfeld, Christian, Richter, Joachim, Hawgood, Samuel, Reid, Kenneth B., Clark, Howard and Ochs, Matthias (2009) A recombinant fragment of human surfactant protein D lacking the short collagen-like stalk fails to correct morphological alterations in lungs of SP-D deficient mice. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, 292, (2), 183-189. (doi:10.1002/ar.20830).
Download
Full text not available from this repository.
Description/Abstract
Emphysema-like pathology is a characteristic feature of surfactant protein D (SP-D) knock-out mice. Treatment with a recombinant fragment of human SP-D consisting of a short collagen-like stalk (but not the entire collagen-like domain of native SP-D), neck, and carbohydrate recognizing domain (CRD) inhibits development of emphysema-like pathology in SP-D deficient mice. On the other hand, it has been shown that the entire collagen-like domain is necessary for preventing SP-D knock-out mice from pulmonary emphysema development. Thus, in the present study, we aimed to elucidate the role of the short collagen-like stalk for the function of the recombinant fragment of human SP-D. We treated SP-D knock-out mice with a fragment of human SP-D lacking the short collagen-like stalk and compared the effects on lung morphology with results from untreated wild-type and SP-D knock-out mice and from SP-D knock-out mice treated with a recombinant fragment of human SP-D including the short collagen-like stalk. The fragment of SP-D lacking the short collagen-like stalk failed to correct pulmonary emphysematous alterations demonstrating the importance of the short collagen-like stalk for the biological activity of the recombinant fragment of human SP-D
| Item Type: | Article |
|---|---|
| ISSNs: | 1932-8486 (print) |
| Related URLs: | |
| Keywords: | SP-D, surfactant, pulmonary emphysema, rfhSP-D, stereology |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair |
| Item ID: | 72734 |
| Date Deposited: | 22 Feb 2010 |
| Last Modified: | 25 Apr 2013 20:11 |
| Contributors: | Knudsen, Lars (Author) Wucherpfennig, Katharina (Author) Mackay, Rose-Marie (Author) Townsend, Paul (Author) Muhlfeld, Christian (Author) Richter, Joachim (Author) Hawgood, Samuel (Author) Reid, Kenneth B. (Author) Clark, Howard (Author) Ochs, Matthias (Author) |
| Date: | February 2009 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/72734 |
Actions (login required)
 |
View Item |
