Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry

Holmes, David, Pettigrew, David, Reccius, Christian H., Gwyer, James D., van Berkel, Cess, Holloway, Judith, Davies, Donna E. and Morgan, Hywel (2009) Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry. Lab on a Chip, 9, (20), 2881-2889. (doi:10.1039/b910053a).


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Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system

Item Type: Article
Digital Object Identifier (DOI): doi:10.1039/b910053a
ISSNs: 1473-0197 (print)
1473-0189 (electronic)
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
R Medicine > RZ Other systems of medicine
Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Electronics and Computer Science
University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair
ePrint ID: 72744
Accepted Date and Publication Date:
October 2009Published
7 August 2009Made publicly available
Date Deposited: 24 Feb 2010
Last Modified: 31 Mar 2016 13:05
Point of Care Blood Cell Analysis
Funded by: EPSRC (TS/G001405/1)
Led by: Hywel Morgan
1 January 2009 to 31 December 2012
URI: http://eprints.soton.ac.uk/id/eprint/72744

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