Repeated low-dose skin exposure is an effective sensitizing stimulus, a factor to be taken into account in predicting sensitization risk
Repeated low-dose skin exposure is an effective sensitizing stimulus, a factor to be taken into account in predicting sensitization risk
Background Contact sensitization by ingredients in personal products is an important clinical problem. It is not clear how sensitization is induced by the generally low concentrations at which they occur but it might be the result of repeated exposure.
Objectives To compare the strength of contact sensitization induced by a single exposure to 2,4-dinitrochlorobenzene (DNCB) (60 ?g cm?2) or three repeated exposures to a subsensitizing dose (10 ?g cm?2).
Methods Two groups (n = 10) of healthy adult volunteers were randomized to receive either a single patch of DNCB 60 ?g cm?2 or three once-weekly applications to the same site of 10 ?g cm?2 DCNB. Four weeks after the last application, sensitization was quantified by measurement of responses (skinfold thickness) to a graded series of four challenge doses.
Results All the volunteers were sensitized and the strength of the responses was virtually identical between the groups.
Conclusions The same degree of sensitization was induced by three exposures to DNCB 10 ?g cm?2 as by one exposure to 60 ?g cm?2 of DNCB. Thus repeated exposure to low doses of contact sensitizers may increase the sensitizing potency. This must be taken into account in future risk assessments.
594-597
Paramasivan, P.
7a17cf10-d543-4af8-a2b5-b9500362ce22
Lai, C.
29ba48ea-2d38-497f-8cf9-400237f6a3a0
Pickard, C.
e21117b3-6345-4d09-a876-ac9965ec4d6a
Ardern-Jones, M.
7ac43c24-94ab-4d19-ba69-afaa546bec90
Healy, E.
400fc04d-f81a-474a-ae25-7ff894be0ebd
Friedmann, P.S.
d50bac23-f3ec-4493-8fa0-fa126cbeba88
September 2009
Paramasivan, P.
7a17cf10-d543-4af8-a2b5-b9500362ce22
Lai, C.
29ba48ea-2d38-497f-8cf9-400237f6a3a0
Pickard, C.
e21117b3-6345-4d09-a876-ac9965ec4d6a
Ardern-Jones, M.
7ac43c24-94ab-4d19-ba69-afaa546bec90
Healy, E.
400fc04d-f81a-474a-ae25-7ff894be0ebd
Friedmann, P.S.
d50bac23-f3ec-4493-8fa0-fa126cbeba88
Paramasivan, P., Lai, C., Pickard, C., Ardern-Jones, M., Healy, E. and Friedmann, P.S.
(2009)
Repeated low-dose skin exposure is an effective sensitizing stimulus, a factor to be taken into account in predicting sensitization risk.
British Journal of Dermatology, 162 (3), .
(doi:10.1111/j.1365-2133.2009.09489.x).
Abstract
Background Contact sensitization by ingredients in personal products is an important clinical problem. It is not clear how sensitization is induced by the generally low concentrations at which they occur but it might be the result of repeated exposure.
Objectives To compare the strength of contact sensitization induced by a single exposure to 2,4-dinitrochlorobenzene (DNCB) (60 ?g cm?2) or three repeated exposures to a subsensitizing dose (10 ?g cm?2).
Methods Two groups (n = 10) of healthy adult volunteers were randomized to receive either a single patch of DNCB 60 ?g cm?2 or three once-weekly applications to the same site of 10 ?g cm?2 DCNB. Four weeks after the last application, sensitization was quantified by measurement of responses (skinfold thickness) to a graded series of four challenge doses.
Results All the volunteers were sensitized and the strength of the responses was virtually identical between the groups.
Conclusions The same degree of sensitization was induced by three exposures to DNCB 10 ?g cm?2 as by one exposure to 60 ?g cm?2 of DNCB. Thus repeated exposure to low doses of contact sensitizers may increase the sensitizing potency. This must be taken into account in future risk assessments.
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Published date: September 2009
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Local EPrints ID: 73119
URI: http://eprints.soton.ac.uk/id/eprint/73119
ISSN: 0007-0963
PURE UUID: 01d9024d-1aed-4b10-829a-3bca9cc90b58
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Date deposited: 02 Mar 2010
Last modified: 14 Mar 2024 02:52
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Author:
P. Paramasivan
Author:
C. Lai
Author:
C. Pickard
Author:
P.S. Friedmann
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