Comparison of L-serine uptake by human placental microvillous membrane vesicles and placental villous fragments
Brand, A.P., Greenwood, S.L., Bennett, E.J., Godfrey, K.M., Sibley, C.P., Hanson, M.A. and Lewis, R.M. (2009) Comparison of L-serine uptake by human placental microvillous membrane vesicles and placental villous fragments. Placenta, 31, (5), 456-459. (doi:10.1016/j.placenta.2010.01.016).
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Both syncytiotrophoblast microvillous plasma membrane vesicles (MVM) and placental villous fragments are used to characterize the placental uptake of maternal substrate and to investigate changes in uptake associated with pathological conditions. However, the two techniques have not been directly compared. In this study uptake of 14C-l-serine was compared in placental villous fragments and in MVM prepared from the same placentas.
14C-l-serine uptake into MVM vesicles was mediated by System L and System A and smaller unidentified Na+-dependent and Na+-independent components. In villous fragments an unidentified Na+-dependent component mediated the majority of 14C-l-serine uptake followed by System A and System L. The unidentified Na+-independent component of l-serine uptake was not detected in villous fragments.
The ratio of System A activity to System L activity was similar in villous fragments and MVM vesicles. However, the unidentified Na+-dependent component in villous fragments was significantly higher than that in MVM vesicles. This indicates that the main differences in serine uptake mechanisms identified using the two techniques were not due to differences in System A and System L activity but to differences in the unidentified Na+-dependent component.
This study suggests that uptake of l-serine into MVM vesicles and villous fragments via Systems A and L is comparable, but that this is not true for all components of l-serine uptake
|Digital Object Identifier (DOI):||doi:10.1016/j.placenta.2010.01.016|
|Keywords:||amino acid, transport, system a, system l|
|Subjects:||Q Science > QP Physiology
R Medicine > R Medicine (General)
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
|Date Deposited:||04 Mar 2010|
|Last Modified:||27 Mar 2014 18:52|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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