Payne, Matthew S., Goss, Kevin C.W., Connet, Gary J., Kollamparambil, Tanoj, Legg, Julian P., Thwaites, Richard, Ashton, Mark, Puddy, Victoria, Bruce, Kenneth D. and Peacock, Janet L.
Molecular microbiological characterization of pre-term neonates at risk of bronchopulmonary dysplasia.
Pediatric Research (doi:10.1203/PDR.0b013e3181d026c3).
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The role of infection in bronchopulmonary dysplasia (BPD) is unknown. We present an observational study of 55 premature infants born weighing less than 1.3kg within two level III neonatal intensive care units. Endotracheal aspirates (ETA) and nasogastric aspirates (NGA) were studied with denaturing gradient gel electrophoresis (DGGE) profiling to elucidate the total bacterial community and species-specific PCR was used to detect the presence of Mycoplasma hominis, Ureaplasma urealyticum and Ureaplasma parvum. DGGE identified bacterial species in 59% of NGA and ETA samples combined. A diverse range of species were identified including several implicated in pre-term labour. Species-specific PCR identified Mycoplasma hominis in 25% of NGA and 11% of ETA samples. Among the 48 infants surviving to 36 weeks post conceptual age, ordinal logistic regression showed the odds ratio for BPD or death where Ureaplasma was present/absent was 4.80 (95% CI 1.15, 20.13). After adjusting for number of days ventilated, this was reduced to 2.04 (0.41, 10.25). These data demonstrate how the combined use of DGGE and species-specific PCR identifies a high exposure in utero and at the time of birth to bacteria which might be causally related to pre-term delivery and subsequent lung injury.
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