Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling
Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling
Biomarker research has rapidly expanded over recent years aided by the progressive development of research tools, in particular the different multiplex technologies allowing simultaneous measurement of multiple analytes. It is foreseeable that such technology will have an integral role in clinical studies for establishing biomarker profiles of disease status, but validation of the tools is essential to confirm the reliability of their application. More comparable studies between multiplex platforms are required to enable users to determine which of these are best for a particular clinical study, as different platforms will have varying levels of performance for the validation parameters. Comparison of two multiplex platforms, the Luminex® and the Mesoscale Discovery, has been performed to determine their performance for the validation parameters of sensitivity, precision and accuracy for the cytokines IL-2, IL-4, IL-8, IL-10, IL-12, IFN? and TNF?. When measuring high concentrations both platforms show good accuracy (within +/? 25% recovery) with all cytokines except IL-12 for the MSD. At low concentrations, +/? 25% recovery was seen with all cytokines except IL-2 and IL-8 for the Luminex and IL-2 and IL-12 for the MSD. Although quantitative differences are found, relative differences are comparable, and consequently both platforms have been shown to be suitable for analyzing trends in multiple cytokine profiles, with the Luminex having better precision and the Mesoscale Discovery having greater sensitivity.
multiplex, cytokine, validation
55-64
Chowdhury, Ferdousi
0af499d4-17c5-40cf-9426-0d509ab82595
Williams, Anthiny
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Johnson, Peter
3f6068ce-171e-4c2c-aca9-dc9b6a37413f
2009
Chowdhury, Ferdousi
0af499d4-17c5-40cf-9426-0d509ab82595
Williams, Anthiny
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Johnson, Peter
3f6068ce-171e-4c2c-aca9-dc9b6a37413f
Chowdhury, Ferdousi, Williams, Anthiny and Johnson, Peter
(2009)
Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling.
Journal of Immunological Methods, 340 (1), .
(doi:10.1016/j.jim.2008.10.002).
Abstract
Biomarker research has rapidly expanded over recent years aided by the progressive development of research tools, in particular the different multiplex technologies allowing simultaneous measurement of multiple analytes. It is foreseeable that such technology will have an integral role in clinical studies for establishing biomarker profiles of disease status, but validation of the tools is essential to confirm the reliability of their application. More comparable studies between multiplex platforms are required to enable users to determine which of these are best for a particular clinical study, as different platforms will have varying levels of performance for the validation parameters. Comparison of two multiplex platforms, the Luminex® and the Mesoscale Discovery, has been performed to determine their performance for the validation parameters of sensitivity, precision and accuracy for the cytokines IL-2, IL-4, IL-8, IL-10, IL-12, IFN? and TNF?. When measuring high concentrations both platforms show good accuracy (within +/? 25% recovery) with all cytokines except IL-12 for the MSD. At low concentrations, +/? 25% recovery was seen with all cytokines except IL-2 and IL-8 for the Luminex and IL-2 and IL-12 for the MSD. Although quantitative differences are found, relative differences are comparable, and consequently both platforms have been shown to be suitable for analyzing trends in multiple cytokine profiles, with the Luminex having better precision and the Mesoscale Discovery having greater sensitivity.
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Published date: 2009
Keywords:
multiplex, cytokine, validation
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Local EPrints ID: 73503
URI: http://eprints.soton.ac.uk/id/eprint/73503
ISSN: 0022-1759
PURE UUID: d98e1b83-5d2d-422c-93c2-715002451f13
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Date deposited: 08 Mar 2010
Last modified: 14 Mar 2024 02:41
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Author:
Ferdousi Chowdhury
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