Mousepox detected in a research facility: case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum
Mousepox detected in a research facility: case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum
An outbreak of mousepox in a research institution was caused by Ectromelia-contaminated mouse serum that had been used for bone marrow cell culture and the cells subsequently injected into the footpads of mice. The disease initially was diagnosed by identification of gross and microscopic lesions typical for Ectromelia infection, including foci of necrosis in the liver and spleen and eosinophilic intracytoplasmic inclusion bodies in the skin. The source of infection was determined by PCR analysis to be serum obtained from a commercial vendor. To determine whether viral growth in tissue culture was required to induce viral infection, 36 mice (BALB/cJ, C57BL/6J) were experimentally exposed intraperitoneally, intradermally (footpad), or intranasally to contaminated serum or bone marrow cell cultures using the contaminated serum in the culture medium. Mice were euthanized when clinical signs developed or after 12 wk. Necropsy, PCR of spleen, and serum ELISA were performed on all mice. Mice injected with cell cultures and their cage contacts developed mousepox, antibodies to Ectromelia, and lesions, whereas mice injected with serum without cells did not. Mouse antibody production, a tool commonly used to screen biologic materials for viral contamination, failed to detect active Ectromelia contamination in mouse serum.
case studies
180-186
Labelle, Philippe
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Hahn, Nina E.
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Fraser, Jenelle K.
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Kendall, Lonnie V.
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Ziman, Melanie
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James, Edward
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Shastri, Nilabh
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Griffey, Stephen M.
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April 2009
Labelle, Philippe
de850f1b-83b0-410e-ad89-1406384fb4fa
Hahn, Nina E.
18d2b28a-771c-40d9-b6e3-821a07314c7c
Fraser, Jenelle K.
0fb3f503-8661-40ea-a5fe-6e01752dffb7
Kendall, Lonnie V.
c783a421-b82b-4fbc-bbb1-b1d44708578a
Ziman, Melanie
71ff5b1d-d344-40a5-a654-a743add08998
James, Edward
7dc1afb7-d326-4050-89fc-1f4e2a1a19a4
Shastri, Nilabh
8af29105-c59b-4260-bdde-a97c016e0793
Griffey, Stephen M.
e0f46be1-0ab1-4000-9533-4e597b19c81a
Labelle, Philippe, Hahn, Nina E., Fraser, Jenelle K., Kendall, Lonnie V., Ziman, Melanie, James, Edward, Shastri, Nilabh and Griffey, Stephen M.
(2009)
Mousepox detected in a research facility: case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum.
Comparative Medicine, 59 (2), .
Abstract
An outbreak of mousepox in a research institution was caused by Ectromelia-contaminated mouse serum that had been used for bone marrow cell culture and the cells subsequently injected into the footpads of mice. The disease initially was diagnosed by identification of gross and microscopic lesions typical for Ectromelia infection, including foci of necrosis in the liver and spleen and eosinophilic intracytoplasmic inclusion bodies in the skin. The source of infection was determined by PCR analysis to be serum obtained from a commercial vendor. To determine whether viral growth in tissue culture was required to induce viral infection, 36 mice (BALB/cJ, C57BL/6J) were experimentally exposed intraperitoneally, intradermally (footpad), or intranasally to contaminated serum or bone marrow cell cultures using the contaminated serum in the culture medium. Mice were euthanized when clinical signs developed or after 12 wk. Necropsy, PCR of spleen, and serum ELISA were performed on all mice. Mice injected with cell cultures and their cage contacts developed mousepox, antibodies to Ectromelia, and lesions, whereas mice injected with serum without cells did not. Mouse antibody production, a tool commonly used to screen biologic materials for viral contamination, failed to detect active Ectromelia contamination in mouse serum.
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Published date: April 2009
Keywords:
case studies
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Local EPrints ID: 73513
URI: http://eprints.soton.ac.uk/id/eprint/73513
ISSN: 1532-0820
PURE UUID: 96675326-175d-446c-af61-c9b8a053d486
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Date deposited: 08 Mar 2010
Last modified: 09 Jan 2022 03:22
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Contributors
Author:
Philippe Labelle
Author:
Nina E. Hahn
Author:
Jenelle K. Fraser
Author:
Lonnie V. Kendall
Author:
Melanie Ziman
Author:
Nilabh Shastri
Author:
Stephen M. Griffey
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