Regeneration efficiency and genotypic effect of some indica type Bangaldeshi rice (Oryza sativa L.) landraces
Regeneration efficiency and genotypic effect of some indica type Bangaldeshi rice (Oryza sativa L.) landraces
Genotypic effect was observed for embryogenic callus induction and subsequent plant regeneration in 15 indica type Bangladeshi rice (Oryza sativa L.) landraces. Different growth regulators were found to influence callus induction ability and green plant regeneration. The highest frequency (55.6%) of shoot regeneration was observed in Hashikalmi with the supplementary hormone concentrations and combination of 2 mg/l BA, 1 mg/l NAA and 1.5 mg/l Kn in MS. The embryogenic calli derived from mature seeds successfully produced shoots, and rooted well on MS basal salt with IAA. Five landraces which could be utilized in further manipulation, such as transformation and in vitro hybridization, were found to have higher regeneration ability (± 50%). In vitro regenerated plants were successfully established in soil and produced fertile seeds.
mature embryos, genotypic variability, embryogenic callus, plant regeneration, indica rice
33-42
Khalequzzaman, Mohammad
d87075b3-1028-43a6-adb9-1dd61e134a8c
Haq, N.
d59a37ec-54c6-4267-be57-de498ae37c0b
Hoque, Enamul
6cbdda1c-a953-404e-997d-9b06b6142c40
Aditya, Tamal Late
606685c6-a3cb-4325-9244-2e4f9997c1cc
June 2005
Khalequzzaman, Mohammad
d87075b3-1028-43a6-adb9-1dd61e134a8c
Haq, N.
d59a37ec-54c6-4267-be57-de498ae37c0b
Hoque, Enamul
6cbdda1c-a953-404e-997d-9b06b6142c40
Aditya, Tamal Late
606685c6-a3cb-4325-9244-2e4f9997c1cc
Khalequzzaman, Mohammad, Haq, N., Hoque, Enamul and Aditya, Tamal Late
(2005)
Regeneration efficiency and genotypic effect of some indica type Bangaldeshi rice (Oryza sativa L.) landraces.
Plant Tissue Culture & Biotechnology, 15 (1), .
Abstract
Genotypic effect was observed for embryogenic callus induction and subsequent plant regeneration in 15 indica type Bangladeshi rice (Oryza sativa L.) landraces. Different growth regulators were found to influence callus induction ability and green plant regeneration. The highest frequency (55.6%) of shoot regeneration was observed in Hashikalmi with the supplementary hormone concentrations and combination of 2 mg/l BA, 1 mg/l NAA and 1.5 mg/l Kn in MS. The embryogenic calli derived from mature seeds successfully produced shoots, and rooted well on MS basal salt with IAA. Five landraces which could be utilized in further manipulation, such as transformation and in vitro hybridization, were found to have higher regeneration ability (± 50%). In vitro regenerated plants were successfully established in soil and produced fertile seeds.
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Published date: June 2005
Keywords:
mature embryos, genotypic variability, embryogenic callus, plant regeneration, indica rice
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Local EPrints ID: 73959
URI: http://eprints.soton.ac.uk/id/eprint/73959
ISSN: 1817-3721
PURE UUID: fb0dcbcb-c8ef-4bfa-8258-c537a1a4529b
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Date deposited: 11 Mar 2010
Last modified: 10 Dec 2021 16:52
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Contributors
Author:
Mohammad Khalequzzaman
Author:
Enamul Hoque
Author:
Tamal Late Aditya
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