Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C.
Wilson, V.M., Haq, N. and Evans, P.K. (1985) Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C. Plant Science, 41, (1), 61-68. (doi:10.1016/0168-9452(85)90066-4).
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Protoplasts have been isolated from winged bean (Psophocarpus tetragonolobus) suspension culture cells using a 2-mercaptoethanol supplemented enzyme solution. These protoplasts can be induced to divide and by continued proliferation give rise to callus colonies. Shoots have been regenerated from this callus. These shoots can be rooted and subsequently the plantlets develop to maturity.
|Digital Object Identifier (DOI):||doi:10.1016/0168-9452(85)90066-4|
|Keywords:||isolation, culture, protoplasts, plant regeneration, winged bean|
|Subjects:||T Technology > TA Engineering (General). Civil engineering (General)
Q Science > QH Natural history > QH301 Biology
S Agriculture > SB Plant culture
|Divisions:||University Structure - Pre August 2011 > School of Civil Engineering and the Environment
|Date Deposited:||11 Mar 2010|
|Last Modified:||27 Mar 2014 18:54|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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