The University of Southampton
University of Southampton Institutional Repository

Applicability and reliability of gel filtration to study aquatic humic substances revisited; the effects of pH on molecular size distributions

Applicability and reliability of gel filtration to study aquatic humic substances revisited; the effects of pH on molecular size distributions
Applicability and reliability of gel filtration to study aquatic humic substances revisited; the effects of pH on molecular size distributions
The effect of pH on the molecular size distribution of dissolved humic substances (DHS) in surface waters from four different lakes in northern Europe was studied using Sephadex G-100 gel filtration chromatography (GFC). New methods for the analysis of the elution profiles obtained are introduced, employing parallel graphical and statistical approaches. For all samples investigated, the proportion of the DHS eluted in the excluded fraction tended to diminish with decreasing pH. At the same time, the remaining DHS eluted tended to decrease in apparent molecular size with decreasing pH. Analyses of the elution profiles revealed that the apparent conformational flexibility of the DHS in the samples was greatest for the samples of lowest ionic strength. The applicability and reliability of GFC as a tool for the investigation of aquatic humic substances are discussed.
gel filtration, gel chromatograms, humic substances, ph
0959-3330
753-764
Shaw, Peter J.
935dfebf-9fb6-483c-86da-a21dba8c1989
De Haan, Henk
bde49bc5-b4fe-4dfc-8c83-7f6a6a652254
Jones, Rodger I.
7a2e7099-517d-4e0f-bdfe-407504d0ef7c
Shaw, Peter J.
935dfebf-9fb6-483c-86da-a21dba8c1989
De Haan, Henk
bde49bc5-b4fe-4dfc-8c83-7f6a6a652254
Jones, Rodger I.
7a2e7099-517d-4e0f-bdfe-407504d0ef7c

Shaw, Peter J., De Haan, Henk and Jones, Rodger I. (1994) Applicability and reliability of gel filtration to study aquatic humic substances revisited; the effects of pH on molecular size distributions. Environmental Technology, 15 (8), 753-764. (doi:10.1080/09593339409385481).

Record type: Article

Abstract

The effect of pH on the molecular size distribution of dissolved humic substances (DHS) in surface waters from four different lakes in northern Europe was studied using Sephadex G-100 gel filtration chromatography (GFC). New methods for the analysis of the elution profiles obtained are introduced, employing parallel graphical and statistical approaches. For all samples investigated, the proportion of the DHS eluted in the excluded fraction tended to diminish with decreasing pH. At the same time, the remaining DHS eluted tended to decrease in apparent molecular size with decreasing pH. Analyses of the elution profiles revealed that the apparent conformational flexibility of the DHS in the samples was greatest for the samples of lowest ionic strength. The applicability and reliability of GFC as a tool for the investigation of aquatic humic substances are discussed.

This record has no associated files available for download.

More information

Published date: August 1994
Keywords: gel filtration, gel chromatograms, humic substances, ph

Identifiers

Local EPrints ID: 75748
URI: http://eprints.soton.ac.uk/id/eprint/75748
ISSN: 0959-3330
PURE UUID: 63ecc971-08c6-45b4-b78d-97863d129aed
ORCID for Peter J. Shaw: ORCID iD orcid.org/0000-0003-0925-5010

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 14 Mar 2024 02:37

Export record

Altmetrics

Contributors

Author: Peter J. Shaw ORCID iD
Author: Henk De Haan
Author: Rodger I. Jones

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×