Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays
Wilson, Robert, Kremeskotter, Jens, Schiffrin, David J. and Wilkinson, James S. (1996) Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays. Biosensors & Bioelectronics, 11, (8), 805-810. (doi:10.1016/0956-5663(96)85932-X).
Glucose oxidase was covalently attached to an aminosilanized indium tin oxide coated glass wafer with the hydroxysuccinimide ester of pyridyldithiopropionic acid. The wafer was used as the working electrode in the reaction chamber of a flow injection analyser. When a solution of glucose and luminol was passed over the electrode, glucose was oxidized enzymatically and luminol was oxidized electro-chemically. The products of these reactions generated light in proportion to the amount of glucose in the sample. When glucose was assayed in the range 0-10 mM the detection limit was 0.419 mM with a correlation coefficient of 0.9962.
|Digital Object Identifier (DOI):||doi:10.1016/0956-5663(96)85932-X|
|Subjects:||Q Science > QC Physics
T Technology > TK Electrical engineering. Electronics Nuclear engineering
|Divisions:||University Structure - Pre August 2011 > Optoelectronics Research Centre
|Date Deposited:||11 Mar 2010|
|Last Modified:||27 Mar 2014 18:58|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
Actions (login required)