Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays


Wilson, Robert, Kremeskotter, Jens, Schiffrin, David J. and Wilkinson, James S. (1996) Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays. Biosensors & Bioelectronics, 11, (8), 805-810. (doi:10.1016/0956-5663(96)85932-X).

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Description/Abstract

Glucose oxidase was covalently attached to an aminosilanized indium tin oxide coated glass wafer with the hydroxysuccinimide ester of pyridyldithiopropionic acid. The wafer was used as the working electrode in the reaction chamber of a flow injection analyser. When a solution of glucose and luminol was passed over the electrode, glucose was oxidized enzymatically and luminol was oxidized electro-chemically. The products of these reactions generated light in proportion to the amount of glucose in the sample. When glucose was assayed in the range 0-10 mM the detection limit was 0.419 mM with a correlation coefficient of 0.9962.

Item Type: Article
ISSNs: 0956-5663 (print)
1873-4235 (electronic)
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Subjects: Q Science > QC Physics
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions: University Structure - Pre August 2011 > Optoelectronics Research Centre
ePrint ID: 78164
Date Deposited: 11 Mar 2010
Last Modified: 27 Mar 2014 18:58
URI: http://eprints.soton.ac.uk/id/eprint/78164

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