The University of Southampton
University of Southampton Institutional Repository

Detection of nitric oxide release from single neurons in the pond snail, Lymnaea stagnalis

Detection of nitric oxide release from single neurons in the pond snail, Lymnaea stagnalis
Detection of nitric oxide release from single neurons in the pond snail, Lymnaea stagnalis
Multiple film-coated nitric oxide sensors have been fabricated using Nafion and electropolymerized polyeugenol or o-phenylenediamine on 30-?m carbon fiber disk electrodes. This is a rare study that utilizes disk electrodes rather than the widely used protruding tip microelectrodes in order to measure from a biological environment. These electrodes have been used to evaluate the differences in nitric oxide release between two different identified neurons in the pond snail, Lymnaea stagnalis. These results show the first direct measurements of nitric oxide release from individual neurons. The electrodes are very sensitive to nitric oxide with a detection limit of 2.8 nM and a sensitivity of 9.46 nA ?M-1. The sensor was very selective against a variety of neurochemical interferences such as ascorbic acid, uric acid, and catecholamines and secondary oxidation products such as nitrite. Nitric oxide release was measured from the cell bodies of two neurons, the cerebral giant cell (CGC) and the B2 buccal motor neuron, in the intact but isolated CNS. A high-Ca2+/high-K+ stimulus was capable of evoking reproducible release. For a given stimulus, the B2 neuron released more nitric oxide than the CGC neuron; however, both cells were equally suppressed by the NOS inhibitor l-NAME
0003-2700
7643-7648
Patel, Bhavik Anil
de7efff5-f5df-444d-b31d-5cd774ab7c7c
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Parker, Kim H.
9e87c3a6-5f17-4681-ba96-2c62f2817fb5
Yeoman, Mark S.
0f313a79-c8f9-4b0e-8c95-6321ad5c52b2
O'Hare, Danny
7c94a94e-383c-45de-9d89-cba323416c6b
Patel, Bhavik Anil
de7efff5-f5df-444d-b31d-5cd774ab7c7c
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Parker, Kim H.
9e87c3a6-5f17-4681-ba96-2c62f2817fb5
Yeoman, Mark S.
0f313a79-c8f9-4b0e-8c95-6321ad5c52b2
O'Hare, Danny
7c94a94e-383c-45de-9d89-cba323416c6b

Patel, Bhavik Anil, Arundell, Martin, Parker, Kim H., Yeoman, Mark S. and O'Hare, Danny (2006) Detection of nitric oxide release from single neurons in the pond snail, Lymnaea stagnalis. Analytical Chemistry, 78 (22), 7643-7648. (doi:10.1021/ac060863w).

Record type: Article

Abstract

Multiple film-coated nitric oxide sensors have been fabricated using Nafion and electropolymerized polyeugenol or o-phenylenediamine on 30-?m carbon fiber disk electrodes. This is a rare study that utilizes disk electrodes rather than the widely used protruding tip microelectrodes in order to measure from a biological environment. These electrodes have been used to evaluate the differences in nitric oxide release between two different identified neurons in the pond snail, Lymnaea stagnalis. These results show the first direct measurements of nitric oxide release from individual neurons. The electrodes are very sensitive to nitric oxide with a detection limit of 2.8 nM and a sensitivity of 9.46 nA ?M-1. The sensor was very selective against a variety of neurochemical interferences such as ascorbic acid, uric acid, and catecholamines and secondary oxidation products such as nitrite. Nitric oxide release was measured from the cell bodies of two neurons, the cerebral giant cell (CGC) and the B2 buccal motor neuron, in the intact but isolated CNS. A high-Ca2+/high-K+ stimulus was capable of evoking reproducible release. For a given stimulus, the B2 neuron released more nitric oxide than the CGC neuron; however, both cells were equally suppressed by the NOS inhibitor l-NAME

This record has no associated files available for download.

More information

Published date: November 2006

Identifiers

Local EPrints ID: 155861
URI: http://eprints.soton.ac.uk/id/eprint/155861
ISSN: 0003-2700
PURE UUID: 8c96bc38-976c-4e5f-aa97-2a0d0561066e

Catalogue record

Date deposited: 24 Jun 2010 13:22
Last modified: 14 Mar 2024 01:41

Export record

Altmetrics

Contributors

Author: Bhavik Anil Patel
Author: Martin Arundell
Author: Kim H. Parker
Author: Mark S. Yeoman
Author: Danny O'Hare

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×