Polarised light microscopy of human amniotic fluid: a rapid method to assess fetal lung maturity - a pilot study
Polarised light microscopy of human amniotic fluid: a rapid method to assess fetal lung maturity - a pilot study
Particles of pulmonary surfactant can be detected in minute quantities of amniotic fluid; they appear as round birefringent particles of 0.5-3 µm diameter with a characteristic cross of polarisation. Two hundred and five samples of amniotic fluid of various gestational ages were examined microscopically under polarised light. The number of birefringent particles observed per µI of amniotic fluid correlated well with the percentage of phosphatidyl-choline in the sample. There was also good agreement with palmitate determinations, surface tension measurements, the lecithin:sphingomyelin ratio and the clinical data. This technique is simple and rapid and does not require counting surfactant particles.
307-314
Clark, H.W.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Jacobson, W.
a8538e85-8e17-4bab-8cc4-e30e5d2b7563
1991
Clark, H.W.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Jacobson, W.
a8538e85-8e17-4bab-8cc4-e30e5d2b7563
Clark, H.W. and Jacobson, W.
(1991)
Polarised light microscopy of human amniotic fluid: a rapid method to assess fetal lung maturity - a pilot study.
Journal of Obstetrics & Gynaecology, 11 (5), .
(doi:10.3109/01443619109007766).
Abstract
Particles of pulmonary surfactant can be detected in minute quantities of amniotic fluid; they appear as round birefringent particles of 0.5-3 µm diameter with a characteristic cross of polarisation. Two hundred and five samples of amniotic fluid of various gestational ages were examined microscopically under polarised light. The number of birefringent particles observed per µI of amniotic fluid correlated well with the percentage of phosphatidyl-choline in the sample. There was also good agreement with palmitate determinations, surface tension measurements, the lecithin:sphingomyelin ratio and the clinical data. This technique is simple and rapid and does not require counting surfactant particles.
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Published date: 1991
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Local EPrints ID: 158861
URI: http://eprints.soton.ac.uk/id/eprint/158861
ISSN: 0144-3615
PURE UUID: 141c1fdc-3409-465b-9784-87b60ba4253d
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Date deposited: 24 Jun 2010 09:28
Last modified: 14 Mar 2024 01:52
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Author:
H.W. Clark
Author:
W. Jacobson
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