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Generic protease detection technology for monitoring periodontal disease

Generic protease detection technology for monitoring periodontal disease
Generic protease detection technology for monitoring periodontal disease
Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.


0301-7249
37-47
Zheng, Xinwei
e968427b-1b50-4fd6-b424-7c85e976e883
Cook, Joseph P.
ceba0fd8-96fa-452b-b04d-399608490bfd
Watkinson, Michael
7405d50a-0e9d-4b31-8e23-0a3198eb1abd
Yang, Shoufeng
e0018adf-8123-4a54-b8dd-306c10ca48f1
Douglas, Ian
f13f8f08-61bc-4c8b-af5d-1375f1547a1c
Rawlinson, Andrew
8f8a627d-72ef-4f53-9fb3-e67d29b1f20a
Krause, Steffi
be90715c-687d-4579-97e6-d3e52209f874
Zheng, Xinwei
e968427b-1b50-4fd6-b424-7c85e976e883
Cook, Joseph P.
ceba0fd8-96fa-452b-b04d-399608490bfd
Watkinson, Michael
7405d50a-0e9d-4b31-8e23-0a3198eb1abd
Yang, Shoufeng
e0018adf-8123-4a54-b8dd-306c10ca48f1
Douglas, Ian
f13f8f08-61bc-4c8b-af5d-1375f1547a1c
Rawlinson, Andrew
8f8a627d-72ef-4f53-9fb3-e67d29b1f20a
Krause, Steffi
be90715c-687d-4579-97e6-d3e52209f874

Zheng, Xinwei, Cook, Joseph P., Watkinson, Michael, Yang, Shoufeng, Douglas, Ian, Rawlinson, Andrew and Krause, Steffi (2011) Generic protease detection technology for monitoring periodontal disease. Faraday Discussions, 149, 37-47. (doi:10.1039/C005364C).

Record type: Article

Abstract

Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.


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More information

Published date: 2011
Organisations: Engineering Mats & Surface Engineerg Gp

Identifiers

Local EPrints ID: 172045
URI: http://eprints.soton.ac.uk/id/eprint/172045
ISSN: 0301-7249
PURE UUID: 71438bf8-a66c-49c2-bb06-f1ca1b5b66a5
ORCID for Shoufeng Yang: ORCID iD orcid.org/0000-0002-3888-3211

Catalogue record

Date deposited: 24 Jan 2011 11:20
Last modified: 14 Mar 2024 02:28

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Contributors

Author: Xinwei Zheng
Author: Joseph P. Cook
Author: Michael Watkinson
Author: Shoufeng Yang ORCID iD
Author: Ian Douglas
Author: Andrew Rawlinson
Author: Steffi Krause

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