Cold sodium hydroxide/urea based pretreatment of bamboo for bioethanol production: characterization of the cellulose rich fraction
Cold sodium hydroxide/urea based pretreatment of bamboo for bioethanol production: characterization of the cellulose rich fraction
Bamboo (Neosinocalamus affinis) was subjected to successive pretreatments to isolate cellulose rich fractions for further utilization. In this study, the ball-milled bamboo underwent ultrasound treatment in ethanol solution at 20 °C for 0, 5, 15, 35, and 50 min, respectively. Then the samples were dissolved with 7% NaOH/12% urea solutions at ?12 °C, followed by successive extractions with dioxane, ethanol, and dimethyl sulfoxide (DMSO). The yields of the obtained cellulose rich fractions ranged from 75.1 to 77.7%. In addition, the structural features of the cellulose rich fractions and the original ball-milled sample were comparatively characterized with a set of spectroscopy and wet chemistry methods including FT-IR, CP/MAS 13C NMR, XRD, SEM, sugar analysis, etc. The results showed that the successive pretreatments resulted in partial removal of carbohydrates and lignin. The lignin released during the pretreatments could facilitate the removal of lignin, which was mainly composed of G and S types. The removal of acetyl groups suggested a decrease of the satirical barrier of enzyme absorption to cellulose. The cellulose rich fractions appeared looser and exhibited more internal surfaces compared to the ball-milled sample. The changes of chemical structures and morphology of the treated sample suggested that the cold sodium hydroxide/urea based pretreatments effectively disrupted the recalcitrance of bamboo, generating highly reactive cellulosic materials for enzymatic hydrolysis to produce bioethanol
551-559
Li, Ming-Fei
ab106ef3-4dc0-4398-ab78-be165835a468
Fan, Yong-Ming
f7f08d11-a463-4e8e-b51d-acc9ff942d48
Xu, Feng
f0b3dd0b-beba-4e7f-88d2-eabdf5563d0b
Sun, Run-Cang
a260a89f-0be9-4957-aed2-4872edb16b12
Zhang, Xun-Li
d7cf1181-3276-4da1-9150-e212b333abb1
November 2010
Li, Ming-Fei
ab106ef3-4dc0-4398-ab78-be165835a468
Fan, Yong-Ming
f7f08d11-a463-4e8e-b51d-acc9ff942d48
Xu, Feng
f0b3dd0b-beba-4e7f-88d2-eabdf5563d0b
Sun, Run-Cang
a260a89f-0be9-4957-aed2-4872edb16b12
Zhang, Xun-Li
d7cf1181-3276-4da1-9150-e212b333abb1
Li, Ming-Fei, Fan, Yong-Ming, Xu, Feng, Sun, Run-Cang and Zhang, Xun-Li
(2010)
Cold sodium hydroxide/urea based pretreatment of bamboo for bioethanol production: characterization of the cellulose rich fraction.
Industrial Crops and Products, 32 (3), .
(doi:10.1016/j.indcrop.2010.07.004).
Abstract
Bamboo (Neosinocalamus affinis) was subjected to successive pretreatments to isolate cellulose rich fractions for further utilization. In this study, the ball-milled bamboo underwent ultrasound treatment in ethanol solution at 20 °C for 0, 5, 15, 35, and 50 min, respectively. Then the samples were dissolved with 7% NaOH/12% urea solutions at ?12 °C, followed by successive extractions with dioxane, ethanol, and dimethyl sulfoxide (DMSO). The yields of the obtained cellulose rich fractions ranged from 75.1 to 77.7%. In addition, the structural features of the cellulose rich fractions and the original ball-milled sample were comparatively characterized with a set of spectroscopy and wet chemistry methods including FT-IR, CP/MAS 13C NMR, XRD, SEM, sugar analysis, etc. The results showed that the successive pretreatments resulted in partial removal of carbohydrates and lignin. The lignin released during the pretreatments could facilitate the removal of lignin, which was mainly composed of G and S types. The removal of acetyl groups suggested a decrease of the satirical barrier of enzyme absorption to cellulose. The cellulose rich fractions appeared looser and exhibited more internal surfaces compared to the ball-milled sample. The changes of chemical structures and morphology of the treated sample suggested that the cold sodium hydroxide/urea based pretreatments effectively disrupted the recalcitrance of bamboo, generating highly reactive cellulosic materials for enzymatic hydrolysis to produce bioethanol
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Published date: November 2010
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Local EPrints ID: 172255
URI: http://eprints.soton.ac.uk/id/eprint/172255
ISSN: 0926-6690
PURE UUID: e3644c94-3daa-47db-82f9-eef25f3ea68d
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Date deposited: 25 Jan 2011 15:20
Last modified: 14 Mar 2024 02:52
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Author:
Ming-Fei Li
Author:
Yong-Ming Fan
Author:
Feng Xu
Author:
Run-Cang Sun
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