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Cloning, sequence and expression patterns of mouse desmocollin 2 (DSC 2): a cadherin-like adhesion molecule

Cloning, sequence and expression patterns of mouse desmocollin 2 (DSC 2): a cadherin-like adhesion molecule
Cloning, sequence and expression patterns of mouse desmocollin 2 (DSC 2): a cadherin-like adhesion molecule
Desmocollins are cadherin-like adhesion molecules of desmosomes. We have determined the full cDNA sequence of a murine desmocollin, the homologue of human and bovine type 2 desmocollins (DSC2), and studied its tissue distribution and expression in stratified epithelia. An 8.5 day mouse embryo cDNA library was screened yielding overlapping clones which encoded the mouse DSC2. This gene has an open reading frame of 2710 base pairs (bp) encoding a polypeptide of 902 amino acids (aa). The polypeptide comprises a signal peptide, a precursor peptide, and a mature protein of 766 aa having an extracellular domain of 549 aa, a single transmembrane domain and a cytoplasmic domain of 184 aa. Like other desmocollins, murine DSC2 has two products, Dsc2a and Dsc2b, produced by alternative splicing of a 46 bp exon which encodes 11 COOH-terminal aa followed by an in-frame stop codon. Inclusion of this exon forms Dsc2b which is 54 aa shorter than Dsc2a. Mouse Dsc2a shows 75.7% amino acid identity to human and 63.3% identity to bovine Dsc2a. The mouse desmocollin is also homologous to the cadherins; 32.2% to the most closely related typical cadherin, human N-cadherin. DSC2 is ubiquitously expressed in epithelial tissues and the heart of adult mice and from the blastocyst stage of development. In situ hybridization shows that the gene is most strongly expressed suprabasally in stratified epithelia, similar to the expression of bovine DSC2.

0968-7688
229-237
Lorimer, J.E.
4e2cfc95-04b8-435c-969e-fa3aa06d7ef9
Hall, L.S.
7539a561-3c14-4ba0-ae58-916714102688
Clarke, J.P.
545d9361-99a6-45a1-a799-0b4cf4428d7d
Collins, J.E.
be0e66f1-3036-47fa-9d7e-914c48710ba4
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Garrod, D.R.
b20b86ce-d168-4810-989f-7a3e11d122e2
Lorimer, J.E.
4e2cfc95-04b8-435c-969e-fa3aa06d7ef9
Hall, L.S.
7539a561-3c14-4ba0-ae58-916714102688
Clarke, J.P.
545d9361-99a6-45a1-a799-0b4cf4428d7d
Collins, J.E.
be0e66f1-3036-47fa-9d7e-914c48710ba4
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Garrod, D.R.
b20b86ce-d168-4810-989f-7a3e11d122e2

Lorimer, J.E., Hall, L.S., Clarke, J.P., Collins, J.E., Fleming, T.P. and Garrod, D.R. (1994) Cloning, sequence and expression patterns of mouse desmocollin 2 (DSC 2): a cadherin-like adhesion molecule. Molecular Membrane Biology, 11 (4), 229-237. (PMID:7711832)

Record type: Article

Abstract

Desmocollins are cadherin-like adhesion molecules of desmosomes. We have determined the full cDNA sequence of a murine desmocollin, the homologue of human and bovine type 2 desmocollins (DSC2), and studied its tissue distribution and expression in stratified epithelia. An 8.5 day mouse embryo cDNA library was screened yielding overlapping clones which encoded the mouse DSC2. This gene has an open reading frame of 2710 base pairs (bp) encoding a polypeptide of 902 amino acids (aa). The polypeptide comprises a signal peptide, a precursor peptide, and a mature protein of 766 aa having an extracellular domain of 549 aa, a single transmembrane domain and a cytoplasmic domain of 184 aa. Like other desmocollins, murine DSC2 has two products, Dsc2a and Dsc2b, produced by alternative splicing of a 46 bp exon which encodes 11 COOH-terminal aa followed by an in-frame stop codon. Inclusion of this exon forms Dsc2b which is 54 aa shorter than Dsc2a. Mouse Dsc2a shows 75.7% amino acid identity to human and 63.3% identity to bovine Dsc2a. The mouse desmocollin is also homologous to the cadherins; 32.2% to the most closely related typical cadherin, human N-cadherin. DSC2 is ubiquitously expressed in epithelial tissues and the heart of adult mice and from the blastocyst stage of development. In situ hybridization shows that the gene is most strongly expressed suprabasally in stratified epithelia, similar to the expression of bovine DSC2.

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Published date: October 1994

Identifiers

Local EPrints ID: 179807
URI: http://eprints.soton.ac.uk/id/eprint/179807
ISSN: 0968-7688
PURE UUID: 19fb75e9-ebca-4e0b-ac7e-3f3ab63445ca

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Date deposited: 05 Apr 2011 08:13
Last modified: 22 Jul 2022 17:36

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Contributors

Author: J.E. Lorimer
Author: L.S. Hall
Author: J.P. Clarke
Author: J.E. Collins
Author: T.P. Fleming
Author: D.R. Garrod

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