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DELLAs regulate chlorophyll and carotenoid biosynthesis to prevent photooxidative damage during seedling deetiolation in Arabidopsis

DELLAs regulate chlorophyll and carotenoid biosynthesis to prevent photooxidative damage during seedling deetiolation in Arabidopsis
DELLAs regulate chlorophyll and carotenoid biosynthesis to prevent photooxidative damage during seedling deetiolation in Arabidopsis
In plants, light represents an important environmental signal that triggers the production of photosynthetically active chloroplasts. This developmental switch is critical for plant survival because chlorophyll precursors that accumulate in darkness can be extremely destructive when illuminated. Thus, plants have evolved mechanisms to adaptively control plastid development during the transition into light. Here, we report that the gibberellin (GA)-regulated DELLA proteins play a crucial role in the formation of functional chloroplasts during deetiolation. We show that Arabidopsis thaliana DELLAs accumulating in etiolated cotyledons derepress chlorophyll and carotenoid biosynthetic pathways in the dark by repressing the transcriptional activity of the phytochrome-interacting factor proteins. Accordingly, dark-grown GA-deficient ga1-3 mutants (that accumulate DELLAs) display a similar gene expression pattern to wild-type seedlings grown in the light. Consistent with this, ga1-3 seedlings accumulate higher amounts of protochlorophyllide (a phototoxic chlorophyll precursor) in darkness but, surprisingly, are substantially more resistant to photooxidative damage following transfer into light. This is due to the DELLA-dependent upregulation of the photoprotective enzyme protochlorophyllide oxidoreductase (POR) in the dark. Our results emphasize the role of DELLAs in regulating the levels of POR, protochlorophyllide, and carotenoids in the dark and in protecting etiolated seedlings against photooxidative damage during initial light exposure
1040-4651
1849-1860
Cheminant, Soizic
67016b87-4463-4a14-840b-5b0a2d7e7d29
Wild, Michael
7ff1cffc-6cbe-43d9-8ac8-f35cfdceecee
Bouvier, Florence
28b957ea-a80a-472c-bbf9-5ff66368ea7b
Pelletier, Sandra
8d8e768a-bd4a-44fa-9774-2830531d7d71
Renou, Jean-Pierre
25933db9-5028-4224-a6c8-c3019c857cfb
Erhardt, Mathieu
e38f5115-198d-4538-92dc-dc79ffd8a1d2
Hayes, Scott
363ac942-2e62-4dd5-bb9e-630386da3368
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Genschik, Pascal
b0d8b174-6e72-4faf-8f32-35f982b41d42
Achard, Patrick
20bdf8da-b985-4ca2-a7b3-b49276563021
Cheminant, Soizic
67016b87-4463-4a14-840b-5b0a2d7e7d29
Wild, Michael
7ff1cffc-6cbe-43d9-8ac8-f35cfdceecee
Bouvier, Florence
28b957ea-a80a-472c-bbf9-5ff66368ea7b
Pelletier, Sandra
8d8e768a-bd4a-44fa-9774-2830531d7d71
Renou, Jean-Pierre
25933db9-5028-4224-a6c8-c3019c857cfb
Erhardt, Mathieu
e38f5115-198d-4538-92dc-dc79ffd8a1d2
Hayes, Scott
363ac942-2e62-4dd5-bb9e-630386da3368
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Genschik, Pascal
b0d8b174-6e72-4faf-8f32-35f982b41d42
Achard, Patrick
20bdf8da-b985-4ca2-a7b3-b49276563021

Cheminant, Soizic, Wild, Michael, Bouvier, Florence, Pelletier, Sandra, Renou, Jean-Pierre, Erhardt, Mathieu, Hayes, Scott, Terry, Matthew J., Genschik, Pascal and Achard, Patrick (2011) DELLAs regulate chlorophyll and carotenoid biosynthesis to prevent photooxidative damage during seedling deetiolation in Arabidopsis. The Plant Cell, 23 (5), 1849-1860. (doi:10.1105/tpc.111.085233).

Record type: Article

Abstract

In plants, light represents an important environmental signal that triggers the production of photosynthetically active chloroplasts. This developmental switch is critical for plant survival because chlorophyll precursors that accumulate in darkness can be extremely destructive when illuminated. Thus, plants have evolved mechanisms to adaptively control plastid development during the transition into light. Here, we report that the gibberellin (GA)-regulated DELLA proteins play a crucial role in the formation of functional chloroplasts during deetiolation. We show that Arabidopsis thaliana DELLAs accumulating in etiolated cotyledons derepress chlorophyll and carotenoid biosynthetic pathways in the dark by repressing the transcriptional activity of the phytochrome-interacting factor proteins. Accordingly, dark-grown GA-deficient ga1-3 mutants (that accumulate DELLAs) display a similar gene expression pattern to wild-type seedlings grown in the light. Consistent with this, ga1-3 seedlings accumulate higher amounts of protochlorophyllide (a phototoxic chlorophyll precursor) in darkness but, surprisingly, are substantially more resistant to photooxidative damage following transfer into light. This is due to the DELLA-dependent upregulation of the photoprotective enzyme protochlorophyllide oxidoreductase (POR) in the dark. Our results emphasize the role of DELLAs in regulating the levels of POR, protochlorophyllide, and carotenoids in the dark and in protecting etiolated seedlings against photooxidative damage during initial light exposure

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Published date: 2011
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 187215
URI: http://eprints.soton.ac.uk/id/eprint/187215
ISSN: 1040-4651
PURE UUID: 52da854e-9940-4262-9145-593efc97afdc
ORCID for Matthew J. Terry: ORCID iD orcid.org/0000-0001-5002-2708

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Date deposited: 16 May 2011 14:06
Last modified: 15 Mar 2024 02:52

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Contributors

Author: Soizic Cheminant
Author: Michael Wild
Author: Florence Bouvier
Author: Sandra Pelletier
Author: Jean-Pierre Renou
Author: Mathieu Erhardt
Author: Scott Hayes
Author: Pascal Genschik
Author: Patrick Achard

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