The University of Southampton
University of Southampton Institutional Repository

Comparison of the distribution pattern of PEG-b-PCL polymersomes delivered into the rat inner ear via different methods

Comparison of the distribution pattern of PEG-b-PCL polymersomes delivered into the rat inner ear via different methods
Comparison of the distribution pattern of PEG-b-PCL polymersomes delivered into the rat inner ear via different methods
Cochleostomy is the most efficient approach in delivering PEG-b-PCL polymersomes (PMs) to the inner ear. PMs 10 can be delivered to the vestibule by transtympanic injection or cochleostomy. Objective: To evaluate the efficiency of delivering 11 PEG-b-PCL PMs into the inner ear using different approaches. Methods: The PEG-b-PCL PMs were administered either by 12 sustained topical round window membrane (RWM) delivery using gelatin sponge pledgets in combination with an osmotic 13 pump, transtympanic injection, or cochleostomy. The distribution of the PMs in the inner ear was observed by confocal 14 microscopy using either whole mount specimens or cryosections. Results: Cochleostomy resulted in distribution of the PMs in 15 the spiral ligament (SL), mesothelial cells beneath the organ of Corti, supporting cells in the organ of Corti, and spiral ganglion 16 cells (SGCs). Transtympanic injection induced uptake of the PMs in the SL and mesothelial cells beneath the organ of Corti. 17 Topical administration showed distribution of the PMs only in the SL. In the vestibulum, transtympanic injection and 18 cochleostomy induced more distribution of the PMs than did topical RWM delivery (p < 0.05, Kruskal-Wallis test).
0001-6489
Zhang, Y.
f812509d-2a3c-41aa-8ba1-68210952d5a6
Zhang, W.
1c80d4f2-4ba8-41f6-85a6-a76a4d65dc9b
Johnston, A.
2c3d6549-ffa2-4e7b-9812-91aadc0adee6
Newman, T.A.
322290cb-2e9c-445d-a047-00b1bea39a25
Pyykko, I.
9a5fa005-7daa-4cdb-9539-925bfc3533e3
Zou, J.
b7f1d06f-cf77-47c1-b7c1-7466c008a212
Zhang, Y.
f812509d-2a3c-41aa-8ba1-68210952d5a6
Zhang, W.
1c80d4f2-4ba8-41f6-85a6-a76a4d65dc9b
Johnston, A.
2c3d6549-ffa2-4e7b-9812-91aadc0adee6
Newman, T.A.
322290cb-2e9c-445d-a047-00b1bea39a25
Pyykko, I.
9a5fa005-7daa-4cdb-9539-925bfc3533e3
Zou, J.
b7f1d06f-cf77-47c1-b7c1-7466c008a212

Zhang, Y., Zhang, W., Johnston, A., Newman, T.A., Pyykko, I. and Zou, J. (2011) Comparison of the distribution pattern of PEG-b-PCL polymersomes delivered into the rat inner ear via different methods. Acta Oto-Laryngologica. (PMID:21905960) (In Press)

Record type: Article

Abstract

Cochleostomy is the most efficient approach in delivering PEG-b-PCL polymersomes (PMs) to the inner ear. PMs 10 can be delivered to the vestibule by transtympanic injection or cochleostomy. Objective: To evaluate the efficiency of delivering 11 PEG-b-PCL PMs into the inner ear using different approaches. Methods: The PEG-b-PCL PMs were administered either by 12 sustained topical round window membrane (RWM) delivery using gelatin sponge pledgets in combination with an osmotic 13 pump, transtympanic injection, or cochleostomy. The distribution of the PMs in the inner ear was observed by confocal 14 microscopy using either whole mount specimens or cryosections. Results: Cochleostomy resulted in distribution of the PMs in 15 the spiral ligament (SL), mesothelial cells beneath the organ of Corti, supporting cells in the organ of Corti, and spiral ganglion 16 cells (SGCs). Transtympanic injection induced uptake of the PMs in the SL and mesothelial cells beneath the organ of Corti. 17 Topical administration showed distribution of the PMs only in the SL. In the vestibulum, transtympanic injection and 18 cochleostomy induced more distribution of the PMs than did topical RWM delivery (p < 0.05, Kruskal-Wallis test).

This record has no associated files available for download.

More information

Accepted/In Press date: 26 July 2011
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 196021
URI: http://eprints.soton.ac.uk/id/eprint/196021
ISSN: 0001-6489
PURE UUID: 8a498f03-c757-4c03-bb8d-443040dbe68b
ORCID for T.A. Newman: ORCID iD orcid.org/0000-0002-3727-9258

Catalogue record

Date deposited: 01 Sep 2011 10:04
Last modified: 18 Oct 2022 01:34

Export record

Contributors

Author: Y. Zhang
Author: W. Zhang
Author: A. Johnston
Author: T.A. Newman ORCID iD
Author: I. Pyykko
Author: J. Zou

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×