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A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens

A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens
A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens
DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.
dna vaccine, neutralizing humoral antibody, t cell help, infectious disease
405-419
Rice, Jason
d58d4fcd-8dc0-4599-bf96-62323d579227
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Rice, Jason
d58d4fcd-8dc0-4599-bf96-62323d579227
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078

Rice, Jason and Christodoulides, Myron (2011) A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens. Methods in Molecular Biology, 799, 405-419. (doi:10.1007/978-1-61779-346-2_22). (PMID:21993657)

Record type: Article

Abstract

DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.

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More information

Published date: 19 October 2011
Keywords: dna vaccine, neutralizing humoral antibody, t cell help, infectious disease
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 200257
URI: http://eprints.soton.ac.uk/id/eprint/200257
DOI: doi:10.1007/978-1-61779-346-2_22
PURE UUID: 46b783bf-a54d-4f4d-b136-28373d94511b
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731

Catalogue record

Date deposited: 24 Oct 2011 14:33
Last modified: 15 Mar 2024 02:39

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Contributors

Author: Jason Rice
Author: Myron Christodoulides ORCID iD

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