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Paneth cell granule depletion in the human small intestine under infective and nutritional stress

Paneth cell granule depletion in the human small intestine under infective and nutritional stress
Paneth cell granule depletion in the human small intestine under infective and nutritional stress
Paneth cells are important contributors to the intestinal antimicrobial barrier through synthesis and release of antimicrobial peptides and proteins. Animal studies indicate that Paneth cell numbers, location and granule morphology are altered by infection and zinc status. We examined human tissue to determine whether Paneth cell numbers, distribution or granule morphology are altered in infective, inflammatory and nutritional disorders.
Archival sections from infective disorders (giardiasis, cryptosporidiosis, HIV, helminth infection) were compared with active inflammatory conditions (coeliac, Crohn's and graft-versus-host diseases) and histologically normal tissues. A subset of tissues was studied by electron microscopy and TUNEL staining for apoptosis. Human defensin-5 (HD5) peptide and mRNA was analysed by immunohistochemistry, in situ hybridization and quantitative reverse transcription polymerase chain reaction. Sections from a tropical population cohort study were then analysed to determine the relationship of granule depletion to infection, nutritional status and plasma zinc concentration.
In HIV-related cryptosporidiosis, but not other disorders, Paneth cells were reduced in number and markedly depleted of granules. Paneth cell granule depletion was associated with reduced HD5 immunoreactivity, but this was not due to apoptosis and there was no reduction in mRNA transcripts. In the tropical population studied, depletion of granules was associated with reduced body mass index, reduced plasma zinc levels and HIV infection. Paneth cell granules in human small intestine may be depleted in response to infective and nutritional stress. We postulate that this is one mechanism through which zinc status influences host susceptibility to intestinal infection.
defensins, hiv, innate immunity, mucosal immunology, paneth cells, zinc
0009-9104
303-309
Kelly, P.
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Feakins, R.
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Domizio, P.
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Murphy, J.L.
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Bevins, C.
75cfd039-07bb-4780-b12a-978748cc5091
Wilson, J.
3f1c94b6-ce87-485d-ab0d-5cde14206d72
McPhail, G.
e4cb6cac-899f-439d-9370-eaec22781ab7
Poulsom, R.
2d9bb2a4-78a7-4f86-97ea-38c40d0e0db9
Dhaliwal, W.
d2dd2f41-4cb0-42bd-9ac3-bc891acac566
Kelly, P.
009a793a-463f-4797-b562-cb1c5c1bbd52
Feakins, R.
29d3a5a9-e3f9-42f3-9b0e-c7ae7af4e8da
Domizio, P.
6b04b514-e503-4cbc-8da6-821133bd3b8b
Murphy, J.L.
99e7863d-f870-438a-b4f5-bec8a2e78ebd
Bevins, C.
75cfd039-07bb-4780-b12a-978748cc5091
Wilson, J.
3f1c94b6-ce87-485d-ab0d-5cde14206d72
McPhail, G.
e4cb6cac-899f-439d-9370-eaec22781ab7
Poulsom, R.
2d9bb2a4-78a7-4f86-97ea-38c40d0e0db9
Dhaliwal, W.
d2dd2f41-4cb0-42bd-9ac3-bc891acac566

Kelly, P., Feakins, R., Domizio, P., Murphy, J.L., Bevins, C., Wilson, J., McPhail, G., Poulsom, R. and Dhaliwal, W. (2004) Paneth cell granule depletion in the human small intestine under infective and nutritional stress. Clinical and Experimental Immunology, 135 (2), 303-309. (doi:10.1111/j.1365-2249.2004.02374.x).

Record type: Article

Abstract

Paneth cells are important contributors to the intestinal antimicrobial barrier through synthesis and release of antimicrobial peptides and proteins. Animal studies indicate that Paneth cell numbers, location and granule morphology are altered by infection and zinc status. We examined human tissue to determine whether Paneth cell numbers, distribution or granule morphology are altered in infective, inflammatory and nutritional disorders.
Archival sections from infective disorders (giardiasis, cryptosporidiosis, HIV, helminth infection) were compared with active inflammatory conditions (coeliac, Crohn's and graft-versus-host diseases) and histologically normal tissues. A subset of tissues was studied by electron microscopy and TUNEL staining for apoptosis. Human defensin-5 (HD5) peptide and mRNA was analysed by immunohistochemistry, in situ hybridization and quantitative reverse transcription polymerase chain reaction. Sections from a tropical population cohort study were then analysed to determine the relationship of granule depletion to infection, nutritional status and plasma zinc concentration.
In HIV-related cryptosporidiosis, but not other disorders, Paneth cells were reduced in number and markedly depleted of granules. Paneth cell granule depletion was associated with reduced HD5 immunoreactivity, but this was not due to apoptosis and there was no reduction in mRNA transcripts. In the tropical population studied, depletion of granules was associated with reduced body mass index, reduced plasma zinc levels and HIV infection. Paneth cell granules in human small intestine may be depleted in response to infective and nutritional stress. We postulate that this is one mechanism through which zinc status influences host susceptibility to intestinal infection.

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More information

Published date: 2004
Keywords: defensins, hiv, innate immunity, mucosal immunology, paneth cells, zinc

Identifiers

Local EPrints ID: 25706
URI: http://eprints.soton.ac.uk/id/eprint/25706
ISSN: 0009-9104
PURE UUID: ef4d5ff1-d457-4ee5-b955-884236180fb2

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Date deposited: 21 Apr 2006
Last modified: 15 Mar 2024 07:04

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Contributors

Author: P. Kelly
Author: R. Feakins
Author: P. Domizio
Author: J.L. Murphy
Author: C. Bevins
Author: J. Wilson
Author: G. McPhail
Author: R. Poulsom
Author: W. Dhaliwal

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