The University of Southampton
University of Southampton Institutional Repository

Cross-species color banding in ten cases of myeloid malignancies with complex karyotypes

Cross-species color banding in ten cases of myeloid malignancies with complex karyotypes
Cross-species color banding in ten cases of myeloid malignancies with complex karyotypes
Cross-species color banding is a multiple-color fluorescence in situ hybridization (FISH) technique using probes developed from other animal species. Hybridization to human metaphases produces color banding patterns specific for each homologous chromosome pair. The technique has been evaluated in a complementary manner with G-banding and chromosome painting in a series of 10 myeloid malignancies with complex or unresolved karyotypes. Color banding detected the majority of chromosomal abnormalities, which had been identified by G-banding and in each case revealed chromosomal changes that G-banding had not identified. Painting was necessary to confirm these abnormalities due to the limitation of only seven colors in the color-banded karyotype. At the same time, painting fortuitously uncovered cryptic abnormalities in 6 of 10 cases that had not been detected by color banding. Insertions were visible by painting only. This study has demonstrated that in the analysis of complex karyotypes, the application of color banding revealed the involvement of the long arm of chromosome 3, indicating a poor risk, in two cases not identified by G-banding. Therefore, these techniques applied together have revealed cryptic chromosomal abnormalities with prognostic significance, which in some cases may have implications for patient management.
1045-2257
15-24
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
Yang, Fengtang
5313d0a7-556c-46ba-8b9c-72ffe4afc3f4
Butler, Tina
8baed9ae-1d6c-4486-8252-f9c38eb81df0
Cheung, Kan-Luk
00bac903-f420-42fd-a660-c5d9be52c838
O'Brien, Patricia
b64a0553-aab0-4846-8c51-c94cc0e16815
Hennessy, Brian J.
e96567e9-3e25-4b20-8e24-bc63aeb42844
Prentice, H. Grant
df862642-bc17-4382-85c3-dd07750885d9
Ferguson-Smith, Malcolm
a32c7c29-b5b3-4eee-8c2c-4d9d3000d441
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
Yang, Fengtang
5313d0a7-556c-46ba-8b9c-72ffe4afc3f4
Butler, Tina
8baed9ae-1d6c-4486-8252-f9c38eb81df0
Cheung, Kan-Luk
00bac903-f420-42fd-a660-c5d9be52c838
O'Brien, Patricia
b64a0553-aab0-4846-8c51-c94cc0e16815
Hennessy, Brian J.
e96567e9-3e25-4b20-8e24-bc63aeb42844
Prentice, H. Grant
df862642-bc17-4382-85c3-dd07750885d9
Ferguson-Smith, Malcolm
a32c7c29-b5b3-4eee-8c2c-4d9d3000d441

Harrison, Christine J., Yang, Fengtang, Butler, Tina, Cheung, Kan-Luk, O'Brien, Patricia, Hennessy, Brian J., Prentice, H. Grant and Ferguson-Smith, Malcolm (2001) Cross-species color banding in ten cases of myeloid malignancies with complex karyotypes. Genes, Chromosomes and Cancer, 30 (1), 15-24. (doi:10.1002/1098-2264(2000)9999:9999<::AID-GCC1061>3.0.CO;2-U).

Record type: Article

Abstract

Cross-species color banding is a multiple-color fluorescence in situ hybridization (FISH) technique using probes developed from other animal species. Hybridization to human metaphases produces color banding patterns specific for each homologous chromosome pair. The technique has been evaluated in a complementary manner with G-banding and chromosome painting in a series of 10 myeloid malignancies with complex or unresolved karyotypes. Color banding detected the majority of chromosomal abnormalities, which had been identified by G-banding and in each case revealed chromosomal changes that G-banding had not identified. Painting was necessary to confirm these abnormalities due to the limitation of only seven colors in the color-banded karyotype. At the same time, painting fortuitously uncovered cryptic abnormalities in 6 of 10 cases that had not been detected by color banding. Insertions were visible by painting only. This study has demonstrated that in the analysis of complex karyotypes, the application of color banding revealed the involvement of the long arm of chromosome 3, indicating a poor risk, in two cases not identified by G-banding. Therefore, these techniques applied together have revealed cryptic chromosomal abnormalities with prognostic significance, which in some cases may have implications for patient management.

This record has no associated files available for download.

More information

Published date: 2001

Identifiers

Local EPrints ID: 26365
URI: http://eprints.soton.ac.uk/id/eprint/26365
ISSN: 1045-2257
PURE UUID: 460fbcb3-c13c-4bf6-a096-197fe8aa3a80

Catalogue record

Date deposited: 24 Apr 2006
Last modified: 15 Mar 2024 07:10

Export record

Altmetrics

Contributors

Author: Christine J. Harrison
Author: Fengtang Yang
Author: Tina Butler
Author: Kan-Luk Cheung
Author: Patricia O'Brien
Author: Brian J. Hennessy
Author: H. Grant Prentice
Author: Malcolm Ferguson-Smith

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×