Controlled delivery of proteins into bilayer lipid membranes on chip
Controlled delivery of proteins into bilayer lipid membranes on chip
The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.
1176-1183
Zagnoni, Michele
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Sandison, Mairi E.
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Marius, Phedra
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Lee, Anthony G.
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Morgan, Hywel
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September 2007
Zagnoni, Michele
abfb96d5-0d3c-4b23-864b-b1212f6d5a42
Sandison, Mairi E.
90477e5a-2af6-44b1-8ea8-ae4515594c60
Marius, Phedra
f5bf8a27-2332-4d04-a45b-4b2677fcc865
Lee, Anthony G.
0891914c-e0e2-4ee1-b43e-1b70eb072d8e
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Zagnoni, Michele, Sandison, Mairi E., Marius, Phedra, Lee, Anthony G. and Morgan, Hywel
(2007)
Controlled delivery of proteins into bilayer lipid membranes on chip.
Lab on a Chip, 7 (9), .
(doi:10.1039/b703818f).
Abstract
The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.
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Controlled_delivery_of_proteins_into_bilayer_lipid_membranes_on_chip-2007.pdf
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Published date: September 2007
Organisations:
Nanoelectronics and Nanotechnology, Electronics & Computer Science, Biological Sciences, Centre for Biological Sciences
Identifiers
Local EPrints ID: 264509
URI: http://eprints.soton.ac.uk/id/eprint/264509
ISSN: 1473-0197
PURE UUID: daee2739-918c-44a0-bd05-17eba005e413
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Date deposited: 15 Sep 2007
Last modified: 15 Mar 2024 03:18
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Contributors
Author:
Michele Zagnoni
Author:
Mairi E. Sandison
Author:
Phedra Marius
Author:
Hywel Morgan
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