Bead-based immunoassays using a micro-chip flow cytometer
Bead-based immunoassays using a micro-chip flow cytometer
A microfabricated flow cytometer has been developed for the analysis of micron-sized polymer beads onto which fluorescently labelled proteins have been immobilised. Fluorescence measurements were made on the beads as they flowed through the chip. Binding of antibodies to surface-immobilised antigens was quantitatively assayed using the device. Particles were focused through a detection zone in the centre of the flow channel using negative dielectrophoresis. Impedance measurements of the particles (at 703 kHz) were used to determine particle size and to trigger capture of the fluorescence signal. Antibody binding was measured by fluorescence at single and dual excitation wavelengths (532 nm and 633 nm). Fluorescence compensation techniques were implemented to correct for spectral overspill between optical detection channels. The data from the microfabricated flow cytometer was shown to be comparable to that of a commercial flow cytometer (BD-FACSAria). Graphical abstract image for this article (ID: b707507n)
1048-1056
Holmes, D.
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She, J.K.
2c724d68-6bd7-4e32-b2a7-4f6b43ec4a0f
Roach, P.L.
ca94060c-4443-482b-af3e-979243488ba9
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
2007
Holmes, D.
0bd269a5-3bab-477c-8bb0-a8711a0aa14b
She, J.K.
2c724d68-6bd7-4e32-b2a7-4f6b43ec4a0f
Roach, P.L.
ca94060c-4443-482b-af3e-979243488ba9
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, D., She, J.K., Roach, P.L. and Morgan, H.
(2007)
Bead-based immunoassays using a micro-chip flow cytometer.
Lab on a Chip, 7 (8), .
(doi:10.1039/b707507n).
Abstract
A microfabricated flow cytometer has been developed for the analysis of micron-sized polymer beads onto which fluorescently labelled proteins have been immobilised. Fluorescence measurements were made on the beads as they flowed through the chip. Binding of antibodies to surface-immobilised antigens was quantitatively assayed using the device. Particles were focused through a detection zone in the centre of the flow channel using negative dielectrophoresis. Impedance measurements of the particles (at 703 kHz) were used to determine particle size and to trigger capture of the fluorescence signal. Antibody binding was measured by fluorescence at single and dual excitation wavelengths (532 nm and 633 nm). Fluorescence compensation techniques were implemented to correct for spectral overspill between optical detection channels. The data from the microfabricated flow cytometer was shown to be comparable to that of a commercial flow cytometer (BD-FACSAria). Graphical abstract image for this article (ID: b707507n)
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Published date: 2007
Organisations:
Chemistry, CBDT, Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 266502
URI: http://eprints.soton.ac.uk/id/eprint/266502
ISSN: 1473-0197
PURE UUID: 1b89096b-481f-445c-8b02-7edccbc95446
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Date deposited: 04 Aug 2008 02:27
Last modified: 15 Mar 2024 03:18
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Author:
D. Holmes
Author:
J.K. She
Author:
P.L. Roach
Author:
H. Morgan
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