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Cerebrospinal fluid apolipoprotein E concentration decreases after traumatic brain injury

Cerebrospinal fluid apolipoprotein E concentration decreases after traumatic brain injury
Cerebrospinal fluid apolipoprotein E concentration decreases after traumatic brain injury
The APOE epsilon4 allele has been associated with unfavorable outcome after several types of acute brain injury, yet the biological mechanisms underlying this observation are poorly understood. Postmortem and experimental brain injury studies suggest the presence of increased amounts of apolipoprotein E (apoE) within the neuropil after acute brain injury. We assayed the concentration of apolipoprotein E in the cerebrospinal fluid (CSF) of non-injured controls and patients with traumatic brain injury (TBI) to determine whether differences exist, and if these differences correlate with injury severity and clinical outcome. CSF apoE and S100B, a marker of injury severity, were measured by enzyme linked immunosorbant assay. CSF was sampled from 27 traumatic brain injury patients (mean age 32, median 25, range 16-65 years) within 3 days of injury, and 28 controls (mean age 40, median 37, range 19-73 years). The TBI patients all had a Glasgow Coma Score (GCS) of less than eight (i.e., severe head injury). Clinical outcome was determined using the Glasgow Outcome Score (GOS). The average concentration of apoE in the CSF of controls was 12.4 mg/L (95% CI: 10.5-14.3 mg/L) and in TBI patients was 3.7 mg/L (95% CI: 2.1-4.1 mg/L; Mann-Whitney: p < 0.0001). In contrast, the concentration of S100B in the CSF of TBI patients was significantly higher than that of controls (Mann-Whitney: p < 0.0001). We speculate that apoE is retained within the parenchyma of the central nervous system in response to injury where in view of previous data, it may have a protective role.
0897-7151
243-250
Kay, Andrew D.
7c403f95-8d6a-4b19-90ab-ea9f1a2cf35b
Petzold, Axel
f7d23dac-63d2-4b0c-a0c1-0c2e60cffdc1
Kerr, Mary
fdcb0e15-ba9a-4668-b8bf-bee718a67b18
Keir, Geoff
d23de5cf-6cb3-4330-9de2-735cc965a8d3
Thompson, Ed J.
ced1b6fc-fde1-4d39-a6fa-2955f6213dd9
Nicoll, James A.R.
88c0685f-000e-4eb7-8f72-f36b4985e8ed
Kay, Andrew D.
7c403f95-8d6a-4b19-90ab-ea9f1a2cf35b
Petzold, Axel
f7d23dac-63d2-4b0c-a0c1-0c2e60cffdc1
Kerr, Mary
fdcb0e15-ba9a-4668-b8bf-bee718a67b18
Keir, Geoff
d23de5cf-6cb3-4330-9de2-735cc965a8d3
Thompson, Ed J.
ced1b6fc-fde1-4d39-a6fa-2955f6213dd9
Nicoll, James A.R.
88c0685f-000e-4eb7-8f72-f36b4985e8ed

Kay, Andrew D., Petzold, Axel, Kerr, Mary, Keir, Geoff, Thompson, Ed J. and Nicoll, James A.R. (2003) Cerebrospinal fluid apolipoprotein E concentration decreases after traumatic brain injury. Journal of Neurotrauma, 20 (3), 243-250.

Record type: Article

Abstract

The APOE epsilon4 allele has been associated with unfavorable outcome after several types of acute brain injury, yet the biological mechanisms underlying this observation are poorly understood. Postmortem and experimental brain injury studies suggest the presence of increased amounts of apolipoprotein E (apoE) within the neuropil after acute brain injury. We assayed the concentration of apolipoprotein E in the cerebrospinal fluid (CSF) of non-injured controls and patients with traumatic brain injury (TBI) to determine whether differences exist, and if these differences correlate with injury severity and clinical outcome. CSF apoE and S100B, a marker of injury severity, were measured by enzyme linked immunosorbant assay. CSF was sampled from 27 traumatic brain injury patients (mean age 32, median 25, range 16-65 years) within 3 days of injury, and 28 controls (mean age 40, median 37, range 19-73 years). The TBI patients all had a Glasgow Coma Score (GCS) of less than eight (i.e., severe head injury). Clinical outcome was determined using the Glasgow Outcome Score (GOS). The average concentration of apoE in the CSF of controls was 12.4 mg/L (95% CI: 10.5-14.3 mg/L) and in TBI patients was 3.7 mg/L (95% CI: 2.1-4.1 mg/L; Mann-Whitney: p < 0.0001). In contrast, the concentration of S100B in the CSF of TBI patients was significantly higher than that of controls (Mann-Whitney: p < 0.0001). We speculate that apoE is retained within the parenchyma of the central nervous system in response to injury where in view of previous data, it may have a protective role.

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Published date: 2003

Identifiers

Local EPrints ID: 27613
URI: http://eprints.soton.ac.uk/id/eprint/27613
ISSN: 0897-7151
PURE UUID: 2cbb8bb7-c1a8-4f81-ad74-bc0fa0da4794
ORCID for James A.R. Nicoll: ORCID iD orcid.org/0000-0002-9444-7246

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Date deposited: 26 Apr 2006
Last modified: 08 Jan 2022 02:55

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Contributors

Author: Andrew D. Kay
Author: Axel Petzold
Author: Mary Kerr
Author: Geoff Keir
Author: Ed J. Thompson

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