Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles
Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles
Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti- inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measuredNP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared.SP-D aggregated NPs, especially following the addition of calcium. rfhSP-D enhanced the co-localisation of NPs to epithelial A459 cells in vitro. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.
963-973
Kendall, M.
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Ding, P
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Mackay, Rose-Marie
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Madsen, Jens
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Deb, R.
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Mackenzie, Z
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Kendall, K.
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Clark, H.W.
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August 2013
Kendall, M.
8fa1da40-2fb8-4524-ba41-ab98aae5ddf4
Ding, P
ea9004ea-4a2e-432c-91dc-0eb40a0def68
Mackay, Rose-Marie
19cf1b92-c65d-4baa-a165-ab630bf77ec3
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Deb, R.
479418a1-9f83-4e8f-905e-74a091829fb1
Mackenzie, Z
1ff933a5-bde0-4a59-91ee-4f13d111792f
Kendall, K.
064717f0-495d-4ac6-988d-5995971dd800
Clark, H.W.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Kendall, M., Ding, P, Mackay, Rose-Marie, Madsen, Jens, Deb, R., Mackenzie, Z, Kendall, K. and Clark, H.W.
(2013)
Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles.
Nanotoxicology, 7 (5), .
(doi:10.3109/17435390.2012.689880).
(PMID:22551051)
Abstract
Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti- inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measuredNP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared.SP-D aggregated NPs, especially following the addition of calcium. rfhSP-D enhanced the co-localisation of NPs to epithelial A459 cells in vitro. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.
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Published date: August 2013
Organisations:
Clinical & Experimental Sciences
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Local EPrints ID: 338828
URI: http://eprints.soton.ac.uk/id/eprint/338828
ISSN: 1743-5390
PURE UUID: e064cead-43ff-452d-b27c-3e4e31c70e4c
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Date deposited: 17 May 2012 14:30
Last modified: 15 Mar 2024 03:29
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Contributors
Author:
M. Kendall
Author:
P Ding
Author:
Rose-Marie Mackay
Author:
Jens Madsen
Author:
R. Deb
Author:
Z Mackenzie
Author:
K. Kendall
Author:
H.W. Clark
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