Tapasin enhances MHC class I peptide presentation according to peptide half-life
Tapasin enhances MHC class I peptide presentation according to peptide half-life
Understanding how peptides are selected for presentation by MHC class I is crucial to vaccination strategies based on cytotoxic T lymphocyte priming. We have studied this selection of the MHC class I peptide repertoire in terms of the presentation of a series of individual peptides with a wide range of binding to MHC class I. This series was expressed as minigenes, and the presentation of each peptide variant was determined with the same MHC class I peptide-specific antibody. In wild-type cells, the hierarchy of presentation followed peptide half-life. This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing-associated ERp57. We demonstrate a key role for tapasin in shaping the MHC class I peptide repertoire, as enhancement of presentation in the presence of tapasin correlated with peptide half-life.
11737-11742
Howarth, Mark
75054a4c-d313-4a69-ba86-8a08104ca300
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Tolstrup, Anne B.
7b46dcbd-6410-41e5-aa23-097a0f7cdeb8
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
10 August 2004
Howarth, Mark
75054a4c-d313-4a69-ba86-8a08104ca300
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Tolstrup, Anne B.
7b46dcbd-6410-41e5-aa23-097a0f7cdeb8
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Howarth, Mark, Williams, Anthony, Tolstrup, Anne B. and Elliott, Tim
(2004)
Tapasin enhances MHC class I peptide presentation according to peptide half-life.
Proceedings of the National Academy of Sciences of the United States of America, 101 (32), .
(doi:10.1073/pnas.0306294101).
(PMID:15286279)
Abstract
Understanding how peptides are selected for presentation by MHC class I is crucial to vaccination strategies based on cytotoxic T lymphocyte priming. We have studied this selection of the MHC class I peptide repertoire in terms of the presentation of a series of individual peptides with a wide range of binding to MHC class I. This series was expressed as minigenes, and the presentation of each peptide variant was determined with the same MHC class I peptide-specific antibody. In wild-type cells, the hierarchy of presentation followed peptide half-life. This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing-associated ERp57. We demonstrate a key role for tapasin in shaping the MHC class I peptide repertoire, as enhancement of presentation in the presence of tapasin correlated with peptide half-life.
Text
2004 Tapasin enhances MHC class I peptide presentation according to peptide half life.pdf
- Version of Record
More information
Published date: 10 August 2004
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 384927
URI: http://eprints.soton.ac.uk/id/eprint/384927
ISSN: 0027-8424
PURE UUID: 64cd0033-ea32-4c42-8c85-05f8984b2988
Catalogue record
Date deposited: 21 Jan 2016 11:02
Last modified: 15 Mar 2024 03:08
Export record
Altmetrics
Contributors
Author:
Mark Howarth
Author:
Anne B. Tolstrup
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics