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Short-term exposure to alcohol increases the permeability of human oral mucosa

Short-term exposure to alcohol increases the permeability of human oral mucosa
Short-term exposure to alcohol increases the permeability of human oral mucosa
OBJECTIVES:?The aim of this study was to evaluate the effect of short-term exposure to ethanol on the permeability barrier properties of human oral mucosa.
MATERIALS AND METHODS:?Permeability constants (Kp× 10?4?cm?min?1) to tritiated water were determined, for untreated human ventral tongue, and following treatment with phosphate-buffered saline (PBS), 5, 15 or 40% ethanol using an in vitro perfusion chamber system. Some samples were also exposed to fluorescent-labelled albumin and examined by fluorescence microscopy. Permeability barrier lipid composition was assessed in treated and untreated mucosa by heat separation, solvent extraction and thin layer chromatography.
RESULTS:?Fifteen per cent ethanol significantly increased mucosal permeability (5.8?±?0.44; P?<?0.05) compared with untreated, PBS and 5% ethanol treated mucosa (4.69?±?0.26, 4.48?±?0.3 and 4.13?±?0.27, respectively). Albumin was restricted to the epithelial surface in control tissue, but extended further through the epithelium and, in some cases, into the connective tissue after treatment with ethanol. Biochemical analysis revealed no significant difference in the epithelial lipid composition of treated and untreated mucosa.
CONCLUSIONS:?These results suggest that short-term exposure to ethanol may act as a permeability enhancer, possibly by causing molecular rearrangement of the permeability barrier, not as a result of lipid extraction.
ethanol, permeability, oral mucosa, albumin
1354-523X
349-354
Howie, N.M.
5ec50c17-8cd3-4f6f-82e5-6749b26a822a
Trigkas, T.K.
1c418910-b21c-4b8e-8ff9-0c2d00f7be7a
Cruchley, A.T.
dc57a91e-afdb-4a1c-bc46-0c941aeed3d9
Wertz, P.W.
bca56725-2e0d-45ae-9899-3d828b1be380
Squier, C.A.
4144a25e-b5e9-4883-ac36-498664a498dd
Williams, D.M.
2f8f81fa-4997-4298-8713-24e6cdf8e96f
Howie, N.M.
5ec50c17-8cd3-4f6f-82e5-6749b26a822a
Trigkas, T.K.
1c418910-b21c-4b8e-8ff9-0c2d00f7be7a
Cruchley, A.T.
dc57a91e-afdb-4a1c-bc46-0c941aeed3d9
Wertz, P.W.
bca56725-2e0d-45ae-9899-3d828b1be380
Squier, C.A.
4144a25e-b5e9-4883-ac36-498664a498dd
Williams, D.M.
2f8f81fa-4997-4298-8713-24e6cdf8e96f

Howie, N.M., Trigkas, T.K., Cruchley, A.T., Wertz, P.W., Squier, C.A. and Williams, D.M. (2001) Short-term exposure to alcohol increases the permeability of human oral mucosa. Oral Diseases, 7 (6), 349-354. (doi:10.1034/j.1601-0825.2001.00731.x).

Record type: Article

Abstract

OBJECTIVES:?The aim of this study was to evaluate the effect of short-term exposure to ethanol on the permeability barrier properties of human oral mucosa.
MATERIALS AND METHODS:?Permeability constants (Kp× 10?4?cm?min?1) to tritiated water were determined, for untreated human ventral tongue, and following treatment with phosphate-buffered saline (PBS), 5, 15 or 40% ethanol using an in vitro perfusion chamber system. Some samples were also exposed to fluorescent-labelled albumin and examined by fluorescence microscopy. Permeability barrier lipid composition was assessed in treated and untreated mucosa by heat separation, solvent extraction and thin layer chromatography.
RESULTS:?Fifteen per cent ethanol significantly increased mucosal permeability (5.8?±?0.44; P?<?0.05) compared with untreated, PBS and 5% ethanol treated mucosa (4.69?±?0.26, 4.48?±?0.3 and 4.13?±?0.27, respectively). Albumin was restricted to the epithelial surface in control tissue, but extended further through the epithelium and, in some cases, into the connective tissue after treatment with ethanol. Biochemical analysis revealed no significant difference in the epithelial lipid composition of treated and untreated mucosa.
CONCLUSIONS:?These results suggest that short-term exposure to ethanol may act as a permeability enhancer, possibly by causing molecular rearrangement of the permeability barrier, not as a result of lipid extraction.

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More information

Published date: November 2001
Keywords: ethanol, permeability, oral mucosa, albumin

Identifiers

Local EPrints ID: 48431
URI: http://eprints.soton.ac.uk/id/eprint/48431
ISSN: 1354-523X
PURE UUID: 4c55583e-823a-4fe0-9b9f-6b64f4068751

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Date deposited: 21 Sep 2007
Last modified: 15 Mar 2024 09:46

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Contributors

Author: N.M. Howie
Author: T.K. Trigkas
Author: A.T. Cruchley
Author: P.W. Wertz
Author: C.A. Squier
Author: D.M. Williams

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