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Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]

Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]
Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]
Background: There is evidence in humans and experimental animals for non-genomic transmission of induced phenotypes between generations. Induction of an altered phenotype in the F1 generation by feeding a protein-restricted (PR) diet to pregnant rats involves altered epigenetic regulation of specific genes. We investigated whether altered epigenetic regulation of hepatic PPARa and glucocorticoid receptor (GR) promoters induced in the F1 generation by prenatal undernutrition is passed to the F2 offspring.
Methods: Rats (F0) were fed a control (18% protein) or PR (9% protein) diet throughout pregnancy and chow during lactation. F1 females were mated and fed chow throughout pregnancy and lactation. F1 and F2 offspring were fed chow. Male offspring from the F1 and F2 generations (n =6 per F0 dietary group) were killed at day 80. Methylation of the hepatic PPARa and GR promoters was determined by methylation-sensitive real-time PCR. mRNA expression was measured by real-time RT-PCR.
Results: Methylation of the hepatic PPARa and GR promoters was lower (8% to 11%, p b0.05) in the F1 and F2 PR offspring. There were trends towards higher PPARa and GR expression in the PR offspring.
0378-3782
p.495
Burdge, G.C.
bbf0edb0-d7e2-4e0b-8ca8-a9710f9a1fca
Slater-Jefferies, J.L.
e46c711a-9d4c-436a-b853-828df69bb4d7
Torrens, C.
15a35713-0651-4249-8227-5901e2cfcd22
Phillips, E.S.
66ddd4cb-19a2-4d08-889b-12f418e6878b
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Burdge, G.C.
bbf0edb0-d7e2-4e0b-8ca8-a9710f9a1fca
Slater-Jefferies, J.L.
e46c711a-9d4c-436a-b853-828df69bb4d7
Torrens, C.
15a35713-0651-4249-8227-5901e2cfcd22
Phillips, E.S.
66ddd4cb-19a2-4d08-889b-12f418e6878b
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc

Burdge, G.C., Slater-Jefferies, J.L., Torrens, C., Phillips, E.S., Hanson, M.A. and Lillycrop, K.A. (2006) Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]. Early Human Development, 82 (8), p.495.

Record type: Article

Abstract

Background: There is evidence in humans and experimental animals for non-genomic transmission of induced phenotypes between generations. Induction of an altered phenotype in the F1 generation by feeding a protein-restricted (PR) diet to pregnant rats involves altered epigenetic regulation of specific genes. We investigated whether altered epigenetic regulation of hepatic PPARa and glucocorticoid receptor (GR) promoters induced in the F1 generation by prenatal undernutrition is passed to the F2 offspring.
Methods: Rats (F0) were fed a control (18% protein) or PR (9% protein) diet throughout pregnancy and chow during lactation. F1 females were mated and fed chow throughout pregnancy and lactation. F1 and F2 offspring were fed chow. Male offspring from the F1 and F2 generations (n =6 per F0 dietary group) were killed at day 80. Methylation of the hepatic PPARa and GR promoters was determined by methylation-sensitive real-time PCR. mRNA expression was measured by real-time RT-PCR.
Results: Methylation of the hepatic PPARa and GR promoters was lower (8% to 11%, p b0.05) in the F1 and F2 PR offspring. There were trends towards higher PPARa and GR expression in the PR offspring.

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More information

Published date: 1 August 2006
Additional Information: Abstracts of the 4th World Congress on Developmental Origins of Health and Disease (DOHaD), 13-16 September 2006, Educatorium, University of Utrecht, The Netherlands
Organisations: Biological Sciences, Medicine

Identifiers

Local EPrints ID: 55884
URI: http://eprints.soton.ac.uk/id/eprint/55884
ISSN: 0378-3782
PURE UUID: 545f11d5-ba27-4841-bbaf-cff3d7aeb91b
ORCID for J.L. Slater-Jefferies: ORCID iD orcid.org/0000-0001-8325-1320
ORCID for E.S. Phillips: ORCID iD orcid.org/0000-0001-5268-4203
ORCID for M.A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for K.A. Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489

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Date deposited: 06 Aug 2008
Last modified: 16 Mar 2024 03:50

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Contributors

Author: G.C. Burdge
Author: C. Torrens
Author: E.S. Phillips ORCID iD
Author: M.A. Hanson ORCID iD
Author: K.A. Lillycrop ORCID iD

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