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Neurites undergoing Wallerian degeneration show an apoptotic-like process with annexin V positive staining and loss of mitochondrial membrane potential

Neurites undergoing Wallerian degeneration show an apoptotic-like process with annexin V positive staining and loss of mitochondrial membrane potential
Neurites undergoing Wallerian degeneration show an apoptotic-like process with annexin V positive staining and loss of mitochondrial membrane potential
Wallerian degeneration, the disintegration of the distal part of an injured axon, is an important event in many neurodegenerative diseases. We studied Wallerian degeneration in dorsal root ganglion (DRG) explants in culture by separating neurites from their cell bodies with a scalpel. The severed neurites showed Annexin V positive staining, that spreads distally with a rate comparable to that of slow axonal transport in intact neurons in vivo. Moreover, the injured neurites showed loss of mitochondrial membrane potential. These features resemble those seen when cells undergo apoptosis. These data contribute to a new understanding of the mechanism of axonal degeneration, have implications for the response of stromal cells in central nervous system (CNS) and raise the prospect of new pharmacological treatments for those neurodegenerative pathologies where the protection of the cell body alone does not alleviate the disease.
Neuron, Wallerian degeneration, Apoptotic-like cell death, Annexin V, Dorsal root ganglia, Axonal degeneration, Neurite transection
0168-0102
161-169
Sievers, C.
c88364c0-53c1-4521-a559-ccf6f2c4dc42
Platt, N.
85f5bff6-a1b9-4b60-acff-828638bcac7c
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Coleman, M.P.
83b02e3e-fc40-4bdc-8a34-a1789aff81a1
Conforti, L.
cf17a522-427f-4a79-84e6-23180f84467f
Sievers, C.
c88364c0-53c1-4521-a559-ccf6f2c4dc42
Platt, N.
85f5bff6-a1b9-4b60-acff-828638bcac7c
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Coleman, M.P.
83b02e3e-fc40-4bdc-8a34-a1789aff81a1
Conforti, L.
cf17a522-427f-4a79-84e6-23180f84467f

Sievers, C., Platt, N., Perry, V.H., Coleman, M.P. and Conforti, L. (2003) Neurites undergoing Wallerian degeneration show an apoptotic-like process with annexin V positive staining and loss of mitochondrial membrane potential. Neuroscience Research, 46 (2), 161-169. (doi:10.1016/S0168-0102(03)00039-7).

Record type: Article

Abstract

Wallerian degeneration, the disintegration of the distal part of an injured axon, is an important event in many neurodegenerative diseases. We studied Wallerian degeneration in dorsal root ganglion (DRG) explants in culture by separating neurites from their cell bodies with a scalpel. The severed neurites showed Annexin V positive staining, that spreads distally with a rate comparable to that of slow axonal transport in intact neurons in vivo. Moreover, the injured neurites showed loss of mitochondrial membrane potential. These features resemble those seen when cells undergo apoptosis. These data contribute to a new understanding of the mechanism of axonal degeneration, have implications for the response of stromal cells in central nervous system (CNS) and raise the prospect of new pharmacological treatments for those neurodegenerative pathologies where the protection of the cell body alone does not alleviate the disease.

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More information

Published date: June 2003
Keywords: Neuron, Wallerian degeneration, Apoptotic-like cell death, Annexin V, Dorsal root ganglia, Axonal degeneration, Neurite transection
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 56350
URI: http://eprints.soton.ac.uk/id/eprint/56350
ISSN: 0168-0102
PURE UUID: 7abba172-0938-41a3-92db-bffdc1d3f0e1

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Date deposited: 08 Aug 2008
Last modified: 15 Mar 2024 11:01

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Contributors

Author: C. Sievers
Author: N. Platt
Author: V.H. Perry
Author: M.P. Coleman
Author: L. Conforti

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