ATP depletion increases phosphorylation of elongation factor eEF2 in adult cardiomyocytes independently of inhibition of mTOR signalling
ATP depletion increases phosphorylation of elongation factor eEF2 in adult cardiomyocytes independently of inhibition of mTOR signalling
Translation elongation consumes a high proportion of cellular energy and can be regulated by phosphorylation of elongation factor eEF2 which inhibits its activity. We have studied the effects of ATP depletion on the phosphorylation of eEF2 in adult rat ventricular cardiomyocytes. Energy depletion rapidly leads to inhibition of protein synthesis and increased phosphorylation of eEF2. Stimulation of the AMP-activated protein kinase also causes increases eEF2 phosphorylation. Only at later times is an effect on mTOR signalling observed. These data suggest that energy depletion leads to inhibition of protein synthesis through phosphorylation of eEF2 independently of inhibition of mTOR signalling.
protein synthesis, elongation, mTOR, ATP, AMP-activated protein kinase, Cardiomyocyte
448-452
McLeod, L.E.
d13a83a4-a29f-48ff-80b2-e4009dae804d
Proud, C.G.
c2cc50f9-4565-4d59-9dfc-aa70b9268a6e
1 November 2002
McLeod, L.E.
d13a83a4-a29f-48ff-80b2-e4009dae804d
Proud, C.G.
c2cc50f9-4565-4d59-9dfc-aa70b9268a6e
McLeod, L.E. and Proud, C.G.
(2002)
ATP depletion increases phosphorylation of elongation factor eEF2 in adult cardiomyocytes independently of inhibition of mTOR signalling.
FEBS Letters, 531 (3), .
Abstract
Translation elongation consumes a high proportion of cellular energy and can be regulated by phosphorylation of elongation factor eEF2 which inhibits its activity. We have studied the effects of ATP depletion on the phosphorylation of eEF2 in adult rat ventricular cardiomyocytes. Energy depletion rapidly leads to inhibition of protein synthesis and increased phosphorylation of eEF2. Stimulation of the AMP-activated protein kinase also causes increases eEF2 phosphorylation. Only at later times is an effect on mTOR signalling observed. These data suggest that energy depletion leads to inhibition of protein synthesis through phosphorylation of eEF2 independently of inhibition of mTOR signalling.
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Submitted date: 27 August 2002
Published date: 1 November 2002
Keywords:
protein synthesis, elongation, mTOR, ATP, AMP-activated protein kinase, Cardiomyocyte
Identifiers
Local EPrints ID: 56672
URI: http://eprints.soton.ac.uk/id/eprint/56672
ISSN: 0014-5793
PURE UUID: 622ab0e4-b3e1-42a3-9976-d1c7f1feac3d
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Date deposited: 07 Aug 2008
Last modified: 08 Jan 2022 07:06
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Contributors
Author:
L.E. McLeod
Author:
C.G. Proud
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