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Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease

Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease
Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease
Background Our aim was to understand why some sera from patients with a broad spectrum of autoimmune diseases or non-autoimmune diseases involving enhanced apoptosis, cell lysis and/or putative secondary autoimmune processes show reactions in the tissue transglutaminase (TGc) ELISA used for diagnosis of gluten-sensitive disease.
Methods Sera were compared from groups of patients with autoimmune diseases, diseases involving organ specific enhanced cell death, celiac disease or dermatitis herpetiformis, diseases of non-autoimmune origin, and a group without known disease. IgA antibodies against TGc were detected using human antigen (produced recombinantly in bacterial or human cells) in different systems (non-commercial ELISA with buffers of differing NaCl concentrations, and anti-TGc sandwich ELISA). Anti-gliadin and anti-endomysium antibodies were also determined.
Results Many sera from patients with autoimmune disorders gave a positive signal in the human TGc ELISAs. The signal appeared related to minor impurities in the recombinant human TGc used and to raised serum IgA antibody levels rather than to the occurrence of TGc specific antibodies in these patients.
Conclusions No association of anti-TGc Abs and autoimmune conditions independent of gluten-sensitive disease could be shown. Care should be taken to exclude copurification of chaperones, like heat shock protein 70, where preparing antigens for TGc ELISAs.
Keywords: Tissue transglutaminase; Celiac disease; Gluten-sensitive disease; Autoimmunity; ELISA
tissue transglutaminase, celiac disease, gluten-sensitive disease, autoimmunity, ELISA
0009-8981
126-135
Sardy, M.
e7725514-7ec2-4630-962d-2c0c3c148378
Csikos, M.
a1cf9a6d-c048-4f58-aacd-e727bd982cf3
Geisen, C.
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Preisz, K.
281414f4-a8d8-4978-bfbb-3d1dd84e55ff
Kornsee, Z.
b3215381-7f8a-469c-902b-f613e0d923ab
Tomsits, E.
06bce16f-4007-42f4-bcb2-7115d7335c76
Tox, U.
1cdc19e1-f4b3-4262-a129-57220e8d78e2
Hunzelmann, N.
dcd5445f-4168-4a53-a62d-37954a08ff53
Wieslander, J.
280423a4-d5c8-454f-a16b-ecaf900c6980
Karpati, S.
5f8086b6-966c-4fb2-b820-80722cec7e0c
Paulsson, M.
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Smyth, N.
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Sardy, M.
e7725514-7ec2-4630-962d-2c0c3c148378
Csikos, M.
a1cf9a6d-c048-4f58-aacd-e727bd982cf3
Geisen, C.
e263dcbc-4c4f-4469-acd3-0df69f3f82b6
Preisz, K.
281414f4-a8d8-4978-bfbb-3d1dd84e55ff
Kornsee, Z.
b3215381-7f8a-469c-902b-f613e0d923ab
Tomsits, E.
06bce16f-4007-42f4-bcb2-7115d7335c76
Tox, U.
1cdc19e1-f4b3-4262-a129-57220e8d78e2
Hunzelmann, N.
dcd5445f-4168-4a53-a62d-37954a08ff53
Wieslander, J.
280423a4-d5c8-454f-a16b-ecaf900c6980
Karpati, S.
5f8086b6-966c-4fb2-b820-80722cec7e0c
Paulsson, M.
ada2e3d2-51b9-4a51-b350-15ac7870dc48
Smyth, N.
0eba2a40-3b43-4d40-bb64-621bd7e9d505

Sardy, M., Csikos, M., Geisen, C., Preisz, K., Kornsee, Z., Tomsits, E., Tox, U., Hunzelmann, N., Wieslander, J., Karpati, S., Paulsson, M. and Smyth, N. (2007) Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease. Clinica Chimica Acta, 376 (1-2), 126-135. (doi:10.1016/j.cca.2006.08.006).

Record type: Article

Abstract

Background Our aim was to understand why some sera from patients with a broad spectrum of autoimmune diseases or non-autoimmune diseases involving enhanced apoptosis, cell lysis and/or putative secondary autoimmune processes show reactions in the tissue transglutaminase (TGc) ELISA used for diagnosis of gluten-sensitive disease.
Methods Sera were compared from groups of patients with autoimmune diseases, diseases involving organ specific enhanced cell death, celiac disease or dermatitis herpetiformis, diseases of non-autoimmune origin, and a group without known disease. IgA antibodies against TGc were detected using human antigen (produced recombinantly in bacterial or human cells) in different systems (non-commercial ELISA with buffers of differing NaCl concentrations, and anti-TGc sandwich ELISA). Anti-gliadin and anti-endomysium antibodies were also determined.
Results Many sera from patients with autoimmune disorders gave a positive signal in the human TGc ELISAs. The signal appeared related to minor impurities in the recombinant human TGc used and to raised serum IgA antibody levels rather than to the occurrence of TGc specific antibodies in these patients.
Conclusions No association of anti-TGc Abs and autoimmune conditions independent of gluten-sensitive disease could be shown. Care should be taken to exclude copurification of chaperones, like heat shock protein 70, where preparing antigens for TGc ELISAs.
Keywords: Tissue transglutaminase; Celiac disease; Gluten-sensitive disease; Autoimmunity; ELISA

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Published date: 1 February 2007
Keywords: tissue transglutaminase, celiac disease, gluten-sensitive disease, autoimmunity, ELISA

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Local EPrints ID: 56733
URI: http://eprints.soton.ac.uk/id/eprint/56733
ISSN: 0009-8981
PURE UUID: 62948022-a2c2-4bfe-b975-d8d40f14ab2f

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Date deposited: 08 Aug 2008
Last modified: 15 Mar 2024 11:03

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Contributors

Author: M. Sardy
Author: M. Csikos
Author: C. Geisen
Author: K. Preisz
Author: Z. Kornsee
Author: E. Tomsits
Author: U. Tox
Author: N. Hunzelmann
Author: J. Wieslander
Author: S. Karpati
Author: M. Paulsson
Author: N. Smyth

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