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Deletion and reduced expression of the Fanconi anemia FANCA gene in sporadic acute myeloid leukemia

Deletion and reduced expression of the Fanconi anemia FANCA gene in sporadic acute myeloid leukemia
Deletion and reduced expression of the Fanconi anemia FANCA gene in sporadic acute myeloid leukemia
Fanconi anemia (FA) is an autosomal recessive chromosomal instability disorder caused by mutations in one of seven known genes (FANCA,C,D2,E,F,G and BRCA2). Mutations in the FANCA gene are the most prevalent, accounting for two-thirds of FA cases. Affected individuals have greatly increased risks of acute myeloid leukemia (AML). This raises the question as to whether inherited or acquired mutations in FA genes might be involved in the development of sporadic AML. Quantitative fluorescent PCR was used to screen archival DNA from sporadic AML cases for FANCA deletions, which account for 40% of FANCA mutations in FA homozygotes. Four heterozygous deletions were found in 101 samples screened, which is 35-fold higher than the expected population frequency for germline FANCA deletions (P<0.0001). Sequencing FANCA in the AML samples with FANCA deletions did not detect mutations in the second allele and there was no evidence of epigenetic silencing by hypermethylation. However, real-time quantitative PCR analysis in these samples showed reduced expression of FANCA compared to nondeleted AML samples and to controls. These findings suggest that gene deletions and reduced expression of FANCA may be involved in the promotion of genetic instability in a subset of cases of sporadic AML.
human, metabolism, dna, down-regulation, dna-binding proteins, male, acute disease, gene deletion, fanconi anemia complementation group a protein, sequence deletion, adolescent, homozygote, gene expression regulation, exons, aged, risk, mutation, analysis, genes, genetics, adult, protein, anemia, female, proteins, research support, physiology, leukemia, myeloid, middle aged, non-U.S.gov't, fanconi anemia, London, 80 and over, dna methylation, population, humans, gene silencing, neoplastic, promoter regions (genetics)
0887-6924
420-425
Tischkowitz, M.D.
5aeb0173-e292-434e-9b2f-cca15737913e
Morgan, N.V.
8dbca216-d4f6-49b4-9fc1-9cd75bd8800b
Grimwade, D.
ffb05d2d-ba42-4841-a002-3c80c3b26961
Eddy, C.
853776a5-6723-4f6b-97bd-0f4da3574d29
Ball, S.
e246d210-e85c-47fd-ac94-1b7694e90db2
Vorechovsky, I.
7245de2f-8c9b-4034-8935-9a451d9b682e
Langabeer, S.
66179c64-f31c-4406-a92c-7523fd6ef03e
Stoger, R.
98db781b-24a8-4245-a0f7-fb6804d5bc03
Hodgson, S.V.
bb6bc7e4-8ddd-4fa3-8ce8-a30cd2a19d94
Mathew, C.G.
ebc9a9cf-8b36-461e-90de-93ac1d5ec20d
Tischkowitz, M.D.
5aeb0173-e292-434e-9b2f-cca15737913e
Morgan, N.V.
8dbca216-d4f6-49b4-9fc1-9cd75bd8800b
Grimwade, D.
ffb05d2d-ba42-4841-a002-3c80c3b26961
Eddy, C.
853776a5-6723-4f6b-97bd-0f4da3574d29
Ball, S.
e246d210-e85c-47fd-ac94-1b7694e90db2
Vorechovsky, I.
7245de2f-8c9b-4034-8935-9a451d9b682e
Langabeer, S.
66179c64-f31c-4406-a92c-7523fd6ef03e
Stoger, R.
98db781b-24a8-4245-a0f7-fb6804d5bc03
Hodgson, S.V.
bb6bc7e4-8ddd-4fa3-8ce8-a30cd2a19d94
Mathew, C.G.
ebc9a9cf-8b36-461e-90de-93ac1d5ec20d

Tischkowitz, M.D., Morgan, N.V., Grimwade, D., Eddy, C., Ball, S., Vorechovsky, I., Langabeer, S., Stoger, R., Hodgson, S.V. and Mathew, C.G. (2004) Deletion and reduced expression of the Fanconi anemia FANCA gene in sporadic acute myeloid leukemia. Leukemia, 18 (3), 420-425. (doi:10.1038/sj.leu.2403280).

Record type: Article

Abstract

Fanconi anemia (FA) is an autosomal recessive chromosomal instability disorder caused by mutations in one of seven known genes (FANCA,C,D2,E,F,G and BRCA2). Mutations in the FANCA gene are the most prevalent, accounting for two-thirds of FA cases. Affected individuals have greatly increased risks of acute myeloid leukemia (AML). This raises the question as to whether inherited or acquired mutations in FA genes might be involved in the development of sporadic AML. Quantitative fluorescent PCR was used to screen archival DNA from sporadic AML cases for FANCA deletions, which account for 40% of FANCA mutations in FA homozygotes. Four heterozygous deletions were found in 101 samples screened, which is 35-fold higher than the expected population frequency for germline FANCA deletions (P<0.0001). Sequencing FANCA in the AML samples with FANCA deletions did not detect mutations in the second allele and there was no evidence of epigenetic silencing by hypermethylation. However, real-time quantitative PCR analysis in these samples showed reduced expression of FANCA compared to nondeleted AML samples and to controls. These findings suggest that gene deletions and reduced expression of FANCA may be involved in the promotion of genetic instability in a subset of cases of sporadic AML.

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More information

Published date: 2004
Keywords: human, metabolism, dna, down-regulation, dna-binding proteins, male, acute disease, gene deletion, fanconi anemia complementation group a protein, sequence deletion, adolescent, homozygote, gene expression regulation, exons, aged, risk, mutation, analysis, genes, genetics, adult, protein, anemia, female, proteins, research support, physiology, leukemia, myeloid, middle aged, non-U.S.gov't, fanconi anemia, London, 80 and over, dna methylation, population, humans, gene silencing, neoplastic, promoter regions (genetics)

Identifiers

Local EPrints ID: 60322
URI: http://eprints.soton.ac.uk/id/eprint/60322
ISSN: 0887-6924
PURE UUID: a13de558-a8e8-45dd-ab92-038ac5f16bde
ORCID for I. Vorechovsky: ORCID iD orcid.org/0000-0002-6740-6502

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Date deposited: 05 Sep 2008
Last modified: 16 Mar 2024 03:32

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Contributors

Author: M.D. Tischkowitz
Author: N.V. Morgan
Author: D. Grimwade
Author: C. Eddy
Author: S. Ball
Author: I. Vorechovsky ORCID iD
Author: S. Langabeer
Author: R. Stoger
Author: S.V. Hodgson
Author: C.G. Mathew

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