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Dietary supplementation with fish oil modifies the ability of human monocytes to induce an inflammatory response

Dietary supplementation with fish oil modifies the ability of human monocytes to induce an inflammatory response
Dietary supplementation with fish oil modifies the ability of human monocytes to induce an inflammatory response
Monocytes/macrophages are key orchestrators of inflammation and are involved in the pathogenesis of chronic inflammatory disorders, including atherosclerosis. (n-3) Fatty acids, found in fish oil, have been shown to have protective effects in such disorders. To investigate possible modes of action, we used a monocyte:endothelial cell (EC) coculture model to investigate the pro-inflammatory potential of monocytes. Monocytes were isolated from the blood of donors with peripheral arterial disease (PAD) or control donors, before and after a 12-wk supplementation of their diet with fish oil. The monocytes were cultured with human umbilical vein EC (HUVEC) for 24 h, after which the ability of the HUVEC to recruit flowing neutrophils was tested. Monocytes from either group of donors stimulated the EC to support the adhesion and migration of neutrophils. Fish oil supplementation reduced the potency of monocytes from normal subjects, but not those from patients with PAD, to induce recruitment. Concurrent medication may have acted as a complicating factor. On subgroup analysis, only those free of medication showed a significant effect of fish oil. Responses before or after supplementation were not closely linked to patterns of secretion of cytokines by cultured monocytes, tested in parallel monocultures. These results suggest that fish oil can modulate the ability of monocytes to stimulate EC and that this might contribute to their protective effects against chronic inflammatory disorders. Benefits, however, may depend on existing medical status and on other treatments being received.
0022-3166
2769-2774
Luu, Nguyet-Thin
045dc5c9-25d6-4664-9ea3-d20b31df7a6a
Madden, Jackie
0771e352-d432-41ea-8a7e-4704c1efca46
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Grimble, Robert F.
3100e4d2-8f29-4ca6-a95d-38a6a764865f
Shearman, Cliff P.
cf4d6317-f54d-4ab3-ba49-c6797897bbcf
Chan, Tim
0c447eb1-22b4-4eef-9e3b-d98fa63f949b
Dastur, Neville
0d3a26e5-f429-426e-9b2d-1fb920ad4bd1
Howell, William M.
1c09187d-f560-4432-badc-08c1270ac79d
Rainger, G. Ed
f437d109-e403-40a4-895a-21f740503a86
Nash, Gerard B.
2a1bc801-f2bf-47ba-be93-e259f47fe9c5
Luu, Nguyet-Thin
045dc5c9-25d6-4664-9ea3-d20b31df7a6a
Madden, Jackie
0771e352-d432-41ea-8a7e-4704c1efca46
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Grimble, Robert F.
3100e4d2-8f29-4ca6-a95d-38a6a764865f
Shearman, Cliff P.
cf4d6317-f54d-4ab3-ba49-c6797897bbcf
Chan, Tim
0c447eb1-22b4-4eef-9e3b-d98fa63f949b
Dastur, Neville
0d3a26e5-f429-426e-9b2d-1fb920ad4bd1
Howell, William M.
1c09187d-f560-4432-badc-08c1270ac79d
Rainger, G. Ed
f437d109-e403-40a4-895a-21f740503a86
Nash, Gerard B.
2a1bc801-f2bf-47ba-be93-e259f47fe9c5

Luu, Nguyet-Thin, Madden, Jackie, Calder, Philip C., Grimble, Robert F., Shearman, Cliff P., Chan, Tim, Dastur, Neville, Howell, William M., Rainger, G. Ed and Nash, Gerard B. (2007) Dietary supplementation with fish oil modifies the ability of human monocytes to induce an inflammatory response. Journal of Nutrition, 137 (12), 2769-2774.

Record type: Article

Abstract

Monocytes/macrophages are key orchestrators of inflammation and are involved in the pathogenesis of chronic inflammatory disorders, including atherosclerosis. (n-3) Fatty acids, found in fish oil, have been shown to have protective effects in such disorders. To investigate possible modes of action, we used a monocyte:endothelial cell (EC) coculture model to investigate the pro-inflammatory potential of monocytes. Monocytes were isolated from the blood of donors with peripheral arterial disease (PAD) or control donors, before and after a 12-wk supplementation of their diet with fish oil. The monocytes were cultured with human umbilical vein EC (HUVEC) for 24 h, after which the ability of the HUVEC to recruit flowing neutrophils was tested. Monocytes from either group of donors stimulated the EC to support the adhesion and migration of neutrophils. Fish oil supplementation reduced the potency of monocytes from normal subjects, but not those from patients with PAD, to induce recruitment. Concurrent medication may have acted as a complicating factor. On subgroup analysis, only those free of medication showed a significant effect of fish oil. Responses before or after supplementation were not closely linked to patterns of secretion of cytokines by cultured monocytes, tested in parallel monocultures. These results suggest that fish oil can modulate the ability of monocytes to stimulate EC and that this might contribute to their protective effects against chronic inflammatory disorders. Benefits, however, may depend on existing medical status and on other treatments being received.

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More information

Published date: December 2007
Organisations: Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 61348
URI: http://eprints.soton.ac.uk/id/eprint/61348
ISSN: 0022-3166
PURE UUID: 75fffc9f-0048-4a11-9377-1d253db64a4a
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 23 Sep 2008
Last modified: 23 Jul 2022 01:39

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Contributors

Author: Nguyet-Thin Luu
Author: Jackie Madden
Author: Robert F. Grimble
Author: Tim Chan
Author: Neville Dastur
Author: William M. Howell
Author: G. Ed Rainger
Author: Gerard B. Nash

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