The University of Southampton
University of Southampton Institutional Repository

Chronic surgical site infection due to suture-associated polymicrobial biofilm

Chronic surgical site infection due to suture-associated polymicrobial biofilm
Chronic surgical site infection due to suture-associated polymicrobial biofilm
Background: surgical site infection (SSI) is a common surgical complication; culture-negative SSI presents a particular problem in management.
Methods: examination of explanted foreign bodies (sutures) using confocal laser scanning microscopy (CLSM) and fluorescent in situ hybridization (FISH) after surgical exploration of a chronic culture-negative SSI.
Results: confocal microscopy (CM) demonstrated bacilli and cocci attached to the surface of the explanted sutures in a mixed biofilm. Florescent in situ hybridization confirmed that Staphylococci were components of the mixed biofilm. Removal of the foreign bodies (sutures) resolved the chronic infection.
Conclusion: chronic SSI can arise from underlying bacterial biofilms, which can invest implanted foreign bodies and associated soft tissue surfaces
1096-2964
457-461
Kathju, Sandeep
80cdb7ee-2e0d-4e70-98c9-93682ce05a09
Nistico, Laura
7a83886a-6bf1-46a1-87dd-75a120d41603
Hall-Stoodley, Luanne
94ebdc00-b549-4488-b15f-5310fb965f5b
Post, J. Christopher
832cfa58-9254-4396-8c8f-6fb18cc6c18c
Ehrlich, Garth D.
aa8e5162-77a6-4627-a793-acd724ed0782
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Kathju, Sandeep
80cdb7ee-2e0d-4e70-98c9-93682ce05a09
Nistico, Laura
7a83886a-6bf1-46a1-87dd-75a120d41603
Hall-Stoodley, Luanne
94ebdc00-b549-4488-b15f-5310fb965f5b
Post, J. Christopher
832cfa58-9254-4396-8c8f-6fb18cc6c18c
Ehrlich, Garth D.
aa8e5162-77a6-4627-a793-acd724ed0782
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f

Kathju, Sandeep, Nistico, Laura, Hall-Stoodley, Luanne, Post, J. Christopher, Ehrlich, Garth D. and Stoodley, Paul (2009) Chronic surgical site infection due to suture-associated polymicrobial biofilm. Surgical Infections, 10 (5), 457-461. (doi:10.1089/sur.2008.062).

Record type: Article

Abstract

Background: surgical site infection (SSI) is a common surgical complication; culture-negative SSI presents a particular problem in management.
Methods: examination of explanted foreign bodies (sutures) using confocal laser scanning microscopy (CLSM) and fluorescent in situ hybridization (FISH) after surgical exploration of a chronic culture-negative SSI.
Results: confocal microscopy (CM) demonstrated bacilli and cocci attached to the surface of the explanted sutures in a mixed biofilm. Florescent in situ hybridization confirmed that Staphylococci were components of the mixed biofilm. Removal of the foreign bodies (sutures) resolved the chronic infection.
Conclusion: chronic SSI can arise from underlying bacterial biofilms, which can invest implanted foreign bodies and associated soft tissue surfaces

This record has no associated files available for download.

More information

Published date: November 2009
Additional Information: The National Centre for Advanced Tribology at Southampton (nCATS)

Identifiers

Local EPrints ID: 71638
URI: http://eprints.soton.ac.uk/id/eprint/71638
ISSN: 1096-2964
PURE UUID: b692bc46-b59b-46b4-9acf-30117e62d3fd
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 18 Dec 2009
Last modified: 14 Mar 2024 02:55

Export record

Altmetrics

Contributors

Author: Sandeep Kathju
Author: Laura Nistico
Author: Luanne Hall-Stoodley
Author: J. Christopher Post
Author: Garth D. Ehrlich
Author: Paul Stoodley ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×