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Applying full-field measurement method to Pseudomonas aeruginosa biofilms

Applying full-field measurement method to Pseudomonas aeruginosa biofilms
Applying full-field measurement method to Pseudomonas aeruginosa biofilms
A digital image correlation (DIC) method was applied to characterize the mechanical behavior of Pseudomonas aeruginosa biofilms in response to wall shear stress using digital video micrographs taken from biofilm flow cells. The appearance of the biofilm in the transmitted light photomicrographs presented a natural texture which was highly conducive to random encoding for DIC. The displacement fields were calculated for two biofilm specimens. The DIC method concurred with previous analysis showing that biofilms exhibit viscoelastic behavior, but had the advantage over simple length measurements of longitudinal strain that it could precisely measure local strains in length (x) and width (y) within biofilm clusters with a 2 ?m resolution as a function of time and wall shear stress. It was concluded that DIC was more accurate at measuring elastic moduli than simple length measurements, but that time-lapse 3D images would enable even more accurate estimates to be performed
bacterial biofilm, digital image correlation, mechanical response
0892-7014
695-703
Mathias, J.D.
a36d60b5-808c-4979-a26b-473aa6295c8b
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f
Mathias, J.D.
a36d60b5-808c-4979-a26b-473aa6295c8b
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f

Mathias, J.D. and Stoodley, P. (2009) Applying full-field measurement method to Pseudomonas aeruginosa biofilms. Biofouling, 25 (8), 695-703. (doi:10.1080/08927010903104984).

Record type: Article

Abstract

A digital image correlation (DIC) method was applied to characterize the mechanical behavior of Pseudomonas aeruginosa biofilms in response to wall shear stress using digital video micrographs taken from biofilm flow cells. The appearance of the biofilm in the transmitted light photomicrographs presented a natural texture which was highly conducive to random encoding for DIC. The displacement fields were calculated for two biofilm specimens. The DIC method concurred with previous analysis showing that biofilms exhibit viscoelastic behavior, but had the advantage over simple length measurements of longitudinal strain that it could precisely measure local strains in length (x) and width (y) within biofilm clusters with a 2 ?m resolution as a function of time and wall shear stress. It was concluded that DIC was more accurate at measuring elastic moduli than simple length measurements, but that time-lapse 3D images would enable even more accurate estimates to be performed

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More information

Published date: November 2009
Additional Information: The National Centre for Advanced Tribology at Southampton (nCATS)
Keywords: bacterial biofilm, digital image correlation, mechanical response

Identifiers

Local EPrints ID: 71653
URI: http://eprints.soton.ac.uk/id/eprint/71653
ISSN: 0892-7014
PURE UUID: cfebde8c-35d5-4f93-ae97-9782a2cd6f68
ORCID for P. Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 18 Dec 2009
Last modified: 14 Mar 2024 02:55

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Contributors

Author: J.D. Mathias
Author: P. Stoodley ORCID iD

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