Microgeographic genetic variation of populations of Idotea chelipes (Crustacea: Isopoda) in lagoons of the southern English coast

Jolly, Marc T., Rogers, Alex D. and Sheader, Martin (2003) Microgeographic genetic variation of populations of Idotea chelipes (Crustacea: Isopoda) in lagoons of the southern English coast. Cahiers de Biologie Marine, 44 (4), 319-327.

Abstract

Allozyme variability was examined using starch-gel electrophoresis between sub-populations of Idotea chelipes over a small geographic distance (approx. 104 km), in lagoons within the Solent area (Fort Gilkicker Moat; Ashlett Pond)and at one site in Dorset (the Fleet Lagoon). Genetic identity values indicate that all three populations are conspecific (I = 0.967 to 0.995), and mean heterozygosity per locus for all three populations was 8%. Significant levels of genetic differentiation (mean F sub(ST) = 0.119***; p< 0.001) were detected over this small spatial scale, but this mainly resulted from the slow esterase locus (mean F sub(ST) (no esterase) = 0.072***; p< 0.001). Genetic differentiation was moderate given the low geographic distances between populations with only one to two migrants per deme per generation (N sub(e)m(F sub(ST)) = 1.85) from the Fleet to Gilkicker. There was no significant deviation from the genotypes expected under the Hardy-Weinberg equilibrium despite an overall slight excess of heterozygotes (mean F sub(IS) = -0.01). Pairwise F sub(ST) values indicated that there was limited genetically effective migration between the sampled lagoons. This genetic differentiation may have been promoted by the presence of the Southampton Water and Solent estuarine system that may act as a physical barrier to gene flow for this species. The previous use of the esterase (ET2)locus as a sub-specific biochemical marker for Idotea chelipes is placed in doubt by this study. Original Abstract: La variabilite enzymatique de trois populations lagunaires daIdotea chelipes est examinee sur gel daelectrophorese daamidon a faible echelle spatiale (environ 104 km). Les trois populations font partie de la meme espece (I = 0,967 a 0,995) et laheterozygotie moyenne observee est de 8 %. Des valeurs de differenciation genetique significatives sont observees (F sub(ST)= 0,119***; p< 0,001) bien que resultant principalement de laesterase lente (F sub(ST)(sans esterase) = 0,072*** ; p< 0,001). Etant donnee la faible distance geographique separant les populations, le niveau de differenciation genetique reste modere avec un a deux migrants par deme par generation (Nem(F sub(ST)) = 1,85). En depit daun faible exces en heterozygote (F sub(IS) = -0,01), aucune deviation par rapport a laequilibre de Hardy-Weinberg naest observee. Les valeurs de differenciation genetique entre paires de populations indiquent que les echanges entre lagunes sont limites et que cette differenciation aurait pu etre accentuee par la presence du systeme estuarien oSolento qui jouerait le role de barriere au flux genique pour cette espece. Daautre part, lautilisation prealable de laesterase lente (ET2) comme marqueur subspecifique du complexe Idotea chelipes est placee en doute dans la presente etude.

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