The University of Southampton
University of Southampton Institutional Repository

Comparison of sample storage protocols for the determination of nutrients in natural waters

Record type: Article

There have been several reports on storage protocols for the determination of nutrients in natural waters but each one has been limited to a particular sample matrix and they have reached different, matrix specific conclusions. The aim of this study was therefore to systematically apply the various recommended storage protocols to a range of natural water matrices. Samples from four contrasting sites in the UK, collected in late winter (February, 1999), were filtered and stored under different conditions (?80°C, ?20°C, 4°C and at 4°C and ?20°C with 0.1% (v/v) chloroform) for up to 247 days prior to analysis. The sites were the River Frome in Dorset (a chalk stream catchment) and three sites from the Tamar Estuary (draining a non-chalk catchment) with salinities of 0.5‰, 10‰ and 34‰. Samples and controls were analysed for total oxidised nitrogen (TON) and filterable reactive phosphorus (FRP) using a segmented flow analyser with spectrophotometric detection. To investigate possible seasonal effects (particularly changes in biological and chemical matrix composition), a second sampling campaign was undertaken in early autumn (October, 1999). The results showed that the optimum storage conditions for the determination of TON and FRP were highly matrix dependent, with significant differences in FRP stability between the Frome and Tamar catchments (due to different calcium concentrations) and between samples of different salinities (due to different bacterial populations and/or dissolved organic matter). General guidelines for sample handling and storage are listed and matrix specific recommendations presented for samples rich in calcium and dissolved organic matter.

Full text not available from this repository.

Citation

Gardolinski, P.C.F.C., Hanrahan, G., Achterberg, E.P., Gledhill, M., Tappin, A.D., House, W.A. and Worsfold, P.J. (2001) Comparison of sample storage protocols for the determination of nutrients in natural waters Water Research, 35, pp. 3670-3678. (doi:10.1016/S0043-1354(01)00088-4).

More information

Published date: 2001
Keywords: natural waters, nutrients, storage protocol, nitrate, phosphate, bacteria

Identifiers

Local EPrints ID: 12555
URI: http://eprints.soton.ac.uk/id/eprint/12555
ISSN: 0043-1354
PURE UUID: 11ff8e58-cd50-4c45-aa87-aa1564b88573

Catalogue record

Date deposited: 26 Nov 2004
Last modified: 17 Jul 2017 17:02

Export record

Altmetrics

Contributors

Author: P.C.F.C. Gardolinski
Author: G. Hanrahan
Author: E.P. Achterberg
Author: M. Gledhill
Author: A.D. Tappin
Author: W.A. House
Author: P.J. Worsfold

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×