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Molecular and physiological responses of two classes of marine chromophytic phytoplankton (diatoms and prymnesiophytes) during the development of nutrient-stimulated blooms

Molecular and physiological responses of two classes of marine chromophytic phytoplankton (diatoms and prymnesiophytes) during the development of nutrient-stimulated blooms
Molecular and physiological responses of two classes of marine chromophytic phytoplankton (diatoms and prymnesiophytes) during the development of nutrient-stimulated blooms
Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in the laboratory and also during a 1-month mesocosm experiment in which we investigated the temporal variability in RubisCO gene expression and primary production in response to inorganic nutrient enrichment. We found that the onset of successive bloom events dominated by each of the two classes of chromophyte algae was associated with marked taxon-specific increases in rbcL transcription rates. These observations suggest that measurements of RubisCO gene expression can provide an early indicator of the development of phytoplankton blooms and may also be useful in predicting which taxa are likely to dominate a bloom.
0099-2240
2349-2357
Wyman, M.
d88b7b49-8822-4337-a0b4-a3419b127470
Davies, J.
1b497cbe-bc4e-478c-ae26-4d0020d5e9e5
Crawford, D.W.
1625e56b-d0c8-4c45-b519-094d0e928a63
Purdie, D.A.
18820b32-185a-467a-8019-01f245191cd8
Wyman, M.
d88b7b49-8822-4337-a0b4-a3419b127470
Davies, J.
1b497cbe-bc4e-478c-ae26-4d0020d5e9e5
Crawford, D.W.
1625e56b-d0c8-4c45-b519-094d0e928a63
Purdie, D.A.
18820b32-185a-467a-8019-01f245191cd8

Wyman, M., Davies, J., Crawford, D.W. and Purdie, D.A. (2000) Molecular and physiological responses of two classes of marine chromophytic phytoplankton (diatoms and prymnesiophytes) during the development of nutrient-stimulated blooms. Applied and Environmental Microbiology, 66 (6), 2349-2357.

Record type: Article

Abstract

Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in the laboratory and also during a 1-month mesocosm experiment in which we investigated the temporal variability in RubisCO gene expression and primary production in response to inorganic nutrient enrichment. We found that the onset of successive bloom events dominated by each of the two classes of chromophyte algae was associated with marked taxon-specific increases in rbcL transcription rates. These observations suggest that measurements of RubisCO gene expression can provide an early indicator of the development of phytoplankton blooms and may also be useful in predicting which taxa are likely to dominate a bloom.

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Published date: 2000

Identifiers

Local EPrints ID: 13482
URI: https://eprints.soton.ac.uk/id/eprint/13482
ISSN: 0099-2240
PURE UUID: 20219710-f9f7-4d30-bf87-7012f32819cb

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Date deposited: 08 Dec 2004
Last modified: 17 Jul 2017 17:02

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