Integrated diode detector and optical fibres for in situ detection within micromachined polymerase chain reaction chips
Integrated diode detector and optical fibres for in situ detection within micromachined polymerase chain reaction chips
This paper reports on the design, fabrication, assembly and testing of a micromachined polymerase chain reaction (PCR) system with pigtailed fibre optics. Two major designs have been fabricated and tested. The first design uses two optical fibres, one for the projection of light into the chamber and the other for the collection of the light emitted by a fluorescent dye in the reaction chamber. The second design employs a single optical fibre for the delivery of light into the PCR chamber, with light now being detected using a photodiode integrated into the base and walls of the reaction chamber within the PCR chip. Tests carried out on both systems have produced promising results.
329-333
Schabmueller, C.G.J.
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Pollard, J.R.
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Evans, A.G.R.
9c9500ce-e74b-42fa-9883-a54d036c2901
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Ensell, G.
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Brunnschweiler, A.
2d39ef7c-b95e-476c-b435-316b6618a565
2001
Schabmueller, C.G.J.
aed55c14-478b-47e7-b94c-6c8a6b673773
Pollard, J.R.
0d992947-dd92-472d-b5c1-e4b4f8f19cfe
Evans, A.G.R.
9c9500ce-e74b-42fa-9883-a54d036c2901
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Ensell, G.
48fe0996-1c6b-4816-8bd0-0a3234d36ae8
Brunnschweiler, A.
2d39ef7c-b95e-476c-b435-316b6618a565
Schabmueller, C.G.J., Pollard, J.R., Evans, A.G.R., Wilkinson, J.S., Ensell, G. and Brunnschweiler, A.
(2001)
Integrated diode detector and optical fibres for in situ detection within micromachined polymerase chain reaction chips.
Journal of Micromechanics and Microengineering, 11 (4), .
(doi:10.1088/0960-1317/11/4/307).
Abstract
This paper reports on the design, fabrication, assembly and testing of a micromachined polymerase chain reaction (PCR) system with pigtailed fibre optics. Two major designs have been fabricated and tested. The first design uses two optical fibres, one for the projection of light into the chamber and the other for the collection of the light emitted by a fluorescent dye in the reaction chamber. The second design employs a single optical fibre for the delivery of light into the PCR chamber, with light now being detected using a photodiode integrated into the base and walls of the reaction chamber within the PCR chip. Tests carried out on both systems have produced promising results.
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Published date: 2001
Identifiers
Local EPrints ID: 13665
URI: http://eprints.soton.ac.uk/id/eprint/13665
ISSN: 0960-1317
PURE UUID: ed7d2a10-b5ad-4aa7-8b51-bba86782c1eb
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Date deposited: 12 Jan 2005
Last modified: 16 Mar 2024 02:33
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Contributors
Author:
C.G.J. Schabmueller
Author:
J.R. Pollard
Author:
A.G.R. Evans
Author:
G. Ensell
Author:
A. Brunnschweiler
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