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Design and theoretical evaluation of a novel microfluidic device to be used for PCR

Design and theoretical evaluation of a novel microfluidic device to be used for PCR
Design and theoretical evaluation of a novel microfluidic device to be used for PCR
The design of a novel, microfluidic chip with an integrated micro peristaltic pump and chambers for DNA amplification is described. This chip contains three reaction chambers stable at 90°C, 72°C and 55°C for PCR amplification, a bi-directional peristaltic pump and optical integrated detection of the droplet. A reactant droplet is to be introduced into the device, pumped back and forth between the chambers by the micro peristaltic pump for sample processing. The static behaviour of the micro pump was modelled theoretically in order to evaluate the optimal dimensions for the pump membranes and to obtain the maximum flow rate. Thermal analysis by the finite element method was performed to optimize the location of the heaters and the temperature uniformity over the three reaction chambers. Transient thermal analysis indicates that the reactant droplet can be heated/cooled in the proposed device in less than 1s to achieve the desired temperatures.
S125-S130
Bu, M.
62faacb5-690b-45af-8ee9-0c9378b48abf
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Ensell, G.
48fe0996-1c6b-4816-8bd0-0a3234d36ae8
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Evans, A.G.R.
082f720d-3830-46d7-ba87-b058af733bc3
Bu, M.
62faacb5-690b-45af-8ee9-0c9378b48abf
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Ensell, G.
48fe0996-1c6b-4816-8bd0-0a3234d36ae8
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Evans, A.G.R.
082f720d-3830-46d7-ba87-b058af733bc3

Bu, M., Melvin, T., Ensell, G., Wilkinson, J.S. and Evans, A.G.R. (2003) Design and theoretical evaluation of a novel microfluidic device to be used for PCR. Journal Micromechanics Microengineering, 13 (4), S125-S130. (doi:10.1088/0960-1317/13/4/321).

Record type: Article

Abstract

The design of a novel, microfluidic chip with an integrated micro peristaltic pump and chambers for DNA amplification is described. This chip contains three reaction chambers stable at 90°C, 72°C and 55°C for PCR amplification, a bi-directional peristaltic pump and optical integrated detection of the droplet. A reactant droplet is to be introduced into the device, pumped back and forth between the chambers by the micro peristaltic pump for sample processing. The static behaviour of the micro pump was modelled theoretically in order to evaluate the optimal dimensions for the pump membranes and to obtain the maximum flow rate. Thermal analysis by the finite element method was performed to optimize the location of the heaters and the temperature uniformity over the three reaction chambers. Transient thermal analysis indicates that the reactant droplet can be heated/cooled in the proposed device in less than 1s to achieve the desired temperatures.

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Published date: 2003
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 13843
URI: http://eprints.soton.ac.uk/id/eprint/13843
PURE UUID: be2e9a50-124d-4cfa-bcde-e3b3e8cdca0b
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 12 Jan 2005
Last modified: 07 Oct 2020 02:10

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Contributors

Author: M. Bu
Author: T. Melvin
Author: G. Ensell
Author: J.S. Wilkinson ORCID iD
Author: A.G.R. Evans

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