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Photo-patterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions

Photo-patterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions
Photo-patterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions
A method is presented for the covalent attachment of oligonucleotides to silicon (100) surfaces patterned with micron-scale features. UV light exposure of hydrogen-terminated silicon (100) coated with alkenes functionalized with N-hydroxysuccinimide ester groups results in Si-C bonded monolayers. The N-hydroxysuccinimide ester surfaces act as a template for the subsequent covalent attachment of DNA oligonucleotides. In order to create patterns of surface attached DNA oligonucleotides with high density, the surface attachment chemistry has been investigated and optimised. Micron scale patterning of surfaces was achieved by exposure with UV laser light via a mask. DNA oligonucleotide patterns, with feature sizes of several microns, were reliably produced over large areas. The patterned surfaces were characterised with scanning electron microscopy, epifluorescence microscopy and ellipsometry. Hybridisation with fluorescent label- and gold nanoparticle-conjugates of the complementary oligonucleotide is achieved. The methods offer reliable approaches for the creation of micron-scale motifs of DNA on surfaces.
1-8
Yin, Huabing B.
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Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Greef, Robert
91063544-ed89-4738-b5f4-28a984bf5866
Mailis, Sakellaris
233e0768-3f8d-430e-8fdf-92e6f4f6a0c4
Eason, Rob W.
e38684c3-d18c-41b9-a4aa-def67283b020
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Yin, Huabing B.
2e1000f5-7da9-4164-a3c1-3a81e3359f9b
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Greef, Robert
91063544-ed89-4738-b5f4-28a984bf5866
Mailis, Sakellaris
233e0768-3f8d-430e-8fdf-92e6f4f6a0c4
Eason, Rob W.
e38684c3-d18c-41b9-a4aa-def67283b020
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Yin, Huabing B., Brown, Tom, Greef, Robert, Mailis, Sakellaris, Eason, Rob W., Wilkinson, James S. and Melvin, Tracy (2004) Photo-patterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions. Biophotonics New Frontier: From Genome to Proteome. 27 Apr 2004. pp. 1-8 . (doi:10.1117/12.545736).

Record type: Conference or Workshop Item (Paper)

Abstract

A method is presented for the covalent attachment of oligonucleotides to silicon (100) surfaces patterned with micron-scale features. UV light exposure of hydrogen-terminated silicon (100) coated with alkenes functionalized with N-hydroxysuccinimide ester groups results in Si-C bonded monolayers. The N-hydroxysuccinimide ester surfaces act as a template for the subsequent covalent attachment of DNA oligonucleotides. In order to create patterns of surface attached DNA oligonucleotides with high density, the surface attachment chemistry has been investigated and optimised. Micron scale patterning of surfaces was achieved by exposure with UV laser light via a mask. DNA oligonucleotide patterns, with feature sizes of several microns, were reliably produced over large areas. The patterned surfaces were characterised with scanning electron microscopy, epifluorescence microscopy and ellipsometry. Hybridisation with fluorescent label- and gold nanoparticle-conjugates of the complementary oligonucleotide is achieved. The methods offer reliable approaches for the creation of micron-scale motifs of DNA on surfaces.

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More information

e-pub ahead of print date: September 2004
Additional Information: (SPIE 5461)
Venue - Dates: Biophotonics New Frontier: From Genome to Proteome, 2004-04-27 - 2004-04-27

Identifiers

Local EPrints ID: 13917
URI: https://eprints.soton.ac.uk/id/eprint/13917
PURE UUID: 6cdbb58f-9e97-45c5-9d6b-722931c9a0c9
ORCID for Sakellaris Mailis: ORCID iD orcid.org/0000-0001-8100-2670
ORCID for Rob W. Eason: ORCID iD orcid.org/0000-0001-9704-2204
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 07 Mar 2005
Last modified: 06 Jun 2018 13:19

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Contributors

Author: Huabing B. Yin
Author: Tom Brown
Author: Robert Greef
Author: Rob W. Eason ORCID iD
Author: Tracy Melvin

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