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Flow cytometric analysis of an Emiliana huxleyi bloom terminated by viral infection

Flow cytometric analysis of an Emiliana huxleyi bloom terminated by viral infection
Flow cytometric analysis of an Emiliana huxleyi bloom terminated by viral infection
During a field mesocosm experiment conducted in coastal waters off western Norway, 11 m3 enclosures were filled with unfiltered seawater and enriched daily with different nitrate and phosphate concentrations in order to induce a bloom of the coccolithophorid Emiliana huxleyi under different nutrient regimes.
Flow cytometry (FCM) analysis was performed 5 times d-1 in order to follow the initiation, development and termination of the bloom as well as the production of large virus-like particles (LVLPs) identified as E. huxleyi viruses (EhV). EhV production was observed first in the enclosure where N was in excess, and P limitation induced a lower burst size compared to nitrate-replete enclosures.
These observations suggest a critical role for both P and N in E. huxleyi-EhV interactions. Concomitant to EhV production, a shift was observed between the original population (coccolith-bearing cells) towards a population characterized by the same chlorophyll a (chl a) fluorescence but with lower right angle light scatter values. This population is likely to correspond to either senescent cells losing their coccoliths or cells characterized by a lower production of coccoliths possibly due to viral infection. At the end of experiment, a significant proportion of E. huxleyi had survived after the end of the bloom.
This suggests either the presence of a resistant form of the coccolithophorid or a change in the dominance of different host and/or viral strains during the bloom. A periodical pattern in virus production was recorded with virus number decreasing during the second part of the day suggesting that virus production may be synchronized to the daily light cycle. Our results provide new insights towards the understanding of the relationship between a key marine species and its specific virus.
diel variations, dynamics, emiliana huxleyi, flow cytometry, mesocosms, viruses
0948-3055
111-124
Jacquet, Stéphan
e930aec2-a5f8-42c2-8482-09594cbe77f5
Heldal, Mikal
8bf5589d-2581-453a-9116-a5375ff164c0
Iglesias-Rodriguez, Debora
34da3d8b-ca9d-4db8-91f0-abfed4a5710f
Larsen, Aud
b29b1211-fb3e-4b41-8426-d084b1583343
Wilson, William
bee10bcd-2a96-4a41-88ee-25e2293b15e3
Bratbak, Gunnar
6691386e-4240-4d84-9b45-eb57426fe915
Jacquet, Stéphan
e930aec2-a5f8-42c2-8482-09594cbe77f5
Heldal, Mikal
8bf5589d-2581-453a-9116-a5375ff164c0
Iglesias-Rodriguez, Debora
34da3d8b-ca9d-4db8-91f0-abfed4a5710f
Larsen, Aud
b29b1211-fb3e-4b41-8426-d084b1583343
Wilson, William
bee10bcd-2a96-4a41-88ee-25e2293b15e3
Bratbak, Gunnar
6691386e-4240-4d84-9b45-eb57426fe915

Jacquet, Stéphan, Heldal, Mikal, Iglesias-Rodriguez, Debora, Larsen, Aud, Wilson, William and Bratbak, Gunnar (2002) Flow cytometric analysis of an Emiliana huxleyi bloom terminated by viral infection. Aquatic Microbial Ecology, 27 (2), 111-124. (doi:10.3354/ame027111).

Record type: Article

Abstract

During a field mesocosm experiment conducted in coastal waters off western Norway, 11 m3 enclosures were filled with unfiltered seawater and enriched daily with different nitrate and phosphate concentrations in order to induce a bloom of the coccolithophorid Emiliana huxleyi under different nutrient regimes.
Flow cytometry (FCM) analysis was performed 5 times d-1 in order to follow the initiation, development and termination of the bloom as well as the production of large virus-like particles (LVLPs) identified as E. huxleyi viruses (EhV). EhV production was observed first in the enclosure where N was in excess, and P limitation induced a lower burst size compared to nitrate-replete enclosures.
These observations suggest a critical role for both P and N in E. huxleyi-EhV interactions. Concomitant to EhV production, a shift was observed between the original population (coccolith-bearing cells) towards a population characterized by the same chlorophyll a (chl a) fluorescence but with lower right angle light scatter values. This population is likely to correspond to either senescent cells losing their coccoliths or cells characterized by a lower production of coccoliths possibly due to viral infection. At the end of experiment, a significant proportion of E. huxleyi had survived after the end of the bloom.
This suggests either the presence of a resistant form of the coccolithophorid or a change in the dominance of different host and/or viral strains during the bloom. A periodical pattern in virus production was recorded with virus number decreasing during the second part of the day suggesting that virus production may be synchronized to the daily light cycle. Our results provide new insights towards the understanding of the relationship between a key marine species and its specific virus.

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More information

Published date: 15 March 2002
Additional Information: Originally entered on ePrints with title: Assaying short-time scale dynamics of an Emiliana huxleyi bloom terminated by viral infection.
Keywords: diel variations, dynamics, emiliana huxleyi, flow cytometry, mesocosms, viruses

Identifiers

Local EPrints ID: 13964
URI: http://eprints.soton.ac.uk/id/eprint/13964
ISSN: 0948-3055
PURE UUID: 4bfc26fd-1be6-48dd-b9b6-d5cb4c8b4bd7

Catalogue record

Date deposited: 21 Dec 2004
Last modified: 09 Nov 2021 06:26

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Contributors

Author: Stéphan Jacquet
Author: Mikal Heldal
Author: Debora Iglesias-Rodriguez
Author: Aud Larsen
Author: William Wilson
Author: Gunnar Bratbak

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