The University of Southampton
University of Southampton Institutional Repository

Micro fluidic cytometry for analysis of marine microorganisms

Micro fluidic cytometry for analysis of marine microorganisms
Micro fluidic cytometry for analysis of marine microorganisms
Knowledge of the distribution and concentration of phytoplankton (microscopic algae that live in the oceans) is important, as this reflects and may modify environmentally important events such as pollution, climate change and carbon dioxide sequestration. Optical based flow cytometry has proved to be strategically important for the study of marine environments in general and of phytoplankton in particular because it enables rapid, simultaneous quantitative analysis of multiple optical properties of particles. Unfortunately, cost, complexity and size limit the widespread use of this technique for the study and monitoring of phytoplankton.

The objective of this research was to solve some of the above mentioned problems by developing a small device for deployment on remote underwater vehicles (RUV) for in situ flow cytometric analysis of marine organisms. The core of the system was a lab-on-a-chip (LOC) device with a microfluidic channel with integrated optics and electrodes for the detection of the optical and electrical characteristics of phytoplankton.

The work describes the design, fabrication, characterisation and testing of various LOC devices, together with data and analysis of results. The devices were able to clearly distinguish different populations of phytoplankton and other micro-particles on the basis of their optical and electrical properties. The systems had performance comparable with commercial instruments.

Research also included bio-physical measurements (electro rotation and dielectrophoresis) of marine organisms to allow further modelling of the information obtained from the LOC cytometer.
Benazzi, Giuseppe
0a1faa41-fbc8-426b-a942-f35d44757ebd
Benazzi, Giuseppe
0a1faa41-fbc8-426b-a942-f35d44757ebd
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Mowlem, Matthew C.
6f633ca2-298f-48ee-a025-ce52dd62124f

Benazzi, Giuseppe (2010) Micro fluidic cytometry for analysis of marine microorganisms. University of Southampton, School of Electronics and Computer Science, Doctoral Thesis, 210pp.

Record type: Thesis (Doctoral)

Abstract

Knowledge of the distribution and concentration of phytoplankton (microscopic algae that live in the oceans) is important, as this reflects and may modify environmentally important events such as pollution, climate change and carbon dioxide sequestration. Optical based flow cytometry has proved to be strategically important for the study of marine environments in general and of phytoplankton in particular because it enables rapid, simultaneous quantitative analysis of multiple optical properties of particles. Unfortunately, cost, complexity and size limit the widespread use of this technique for the study and monitoring of phytoplankton.

The objective of this research was to solve some of the above mentioned problems by developing a small device for deployment on remote underwater vehicles (RUV) for in situ flow cytometric analysis of marine organisms. The core of the system was a lab-on-a-chip (LOC) device with a microfluidic channel with integrated optics and electrodes for the detection of the optical and electrical characteristics of phytoplankton.

The work describes the design, fabrication, characterisation and testing of various LOC devices, together with data and analysis of results. The devices were able to clearly distinguish different populations of phytoplankton and other micro-particles on the basis of their optical and electrical properties. The systems had performance comparable with commercial instruments.

Research also included bio-physical measurements (electro rotation and dielectrophoresis) of marine organisms to allow further modelling of the information obtained from the LOC cytometer.

Text
Thesis_FINAL.pdf - Other
Download (8MB)

More information

Published date: January 2010
Organisations: University of Southampton

Identifiers

Local EPrints ID: 141969
URI: http://eprints.soton.ac.uk/id/eprint/141969
PURE UUID: f0cd55d3-c473-4611-824e-7d6ea0cf3a4c
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 14 Jun 2010 15:32
Last modified: 30 Jan 2020 01:32

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×