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Nitric oxide modulates salt and sugar responses via different signaling pathways

Nitric oxide modulates salt and sugar responses via different signaling pathways
Nitric oxide modulates salt and sugar responses via different signaling pathways
Locusts lay their eggs by digging into a substrate using rhythmic opening and closing movements of ovipositor valves at the end of the abdomen. The digging rhythm is inhibited by chemosensory stimulation of chemoreceptors on the valves. Nitric oxide (NO) modulated the effects of chemosensory stimulation on the rhythm. Stimulation with either sucrose or sodium chloride (NaCl) stopped the digging rhythm, whereas simultaneous bath application of the NO inhibitor, N-nitro-L-arginine methyl ester (L-NAME), increased the duration for which the digging rhythm stopped. Increasing NO levels caused a significant reduction in the cessation of the rhythm in response to the same 2 chemicals. Bath applying cyclic guanosine monophosphate (cGMP), the soluble guanylate inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and the generic protein kinase inhibitor H-7 had no effect on the duration for which the rhythm stopped in response to NaCl stimulation. Conversely, bath application of cGMP and ODQ resulted in a significant decrease and increase, respectively, in the duration for which the digging rhythm stopped when stimulated with sucrose. Moreover, bath application of the selective protein kinase G (PKG) inhibitor KT-5823 also resulted in a significant increase in the duration of cessation of the rhythm when stimulated with sucrose. Results suggest that NO modulates the behavioral responses to NaCl via a cGMP/PKG-independent pathway while modulating the responses to sucrose via a NO-cGMP/PKG-dependent pathway.
cGMP, chemoreception, grasshopper, gustation, nitric oxide synthase, oviposition
0379-864X
347-356
Newland, Philip L.
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb
Yates, Paul
8af40dea-121b-4204-affd-86d76a2f5ab1
Newland, Philip L.
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb
Yates, Paul
8af40dea-121b-4204-affd-86d76a2f5ab1

Newland, Philip L. and Yates, Paul (2008) Nitric oxide modulates salt and sugar responses via different signaling pathways. Chemical Senses, 33 (4), 347-356. (doi:10.1093/chemse/bjm094).

Record type: Article

Abstract

Locusts lay their eggs by digging into a substrate using rhythmic opening and closing movements of ovipositor valves at the end of the abdomen. The digging rhythm is inhibited by chemosensory stimulation of chemoreceptors on the valves. Nitric oxide (NO) modulated the effects of chemosensory stimulation on the rhythm. Stimulation with either sucrose or sodium chloride (NaCl) stopped the digging rhythm, whereas simultaneous bath application of the NO inhibitor, N-nitro-L-arginine methyl ester (L-NAME), increased the duration for which the digging rhythm stopped. Increasing NO levels caused a significant reduction in the cessation of the rhythm in response to the same 2 chemicals. Bath applying cyclic guanosine monophosphate (cGMP), the soluble guanylate inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and the generic protein kinase inhibitor H-7 had no effect on the duration for which the rhythm stopped in response to NaCl stimulation. Conversely, bath application of cGMP and ODQ resulted in a significant decrease and increase, respectively, in the duration for which the digging rhythm stopped when stimulated with sucrose. Moreover, bath application of the selective protein kinase G (PKG) inhibitor KT-5823 also resulted in a significant increase in the duration of cessation of the rhythm when stimulated with sucrose. Results suggest that NO modulates the behavioral responses to NaCl via a cGMP/PKG-independent pathway while modulating the responses to sucrose via a NO-cGMP/PKG-dependent pathway.

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More information

Published date: 6 February 2008
Keywords: cGMP, chemoreception, grasshopper, gustation, nitric oxide synthase, oviposition

Identifiers

Local EPrints ID: 142248
URI: http://eprints.soton.ac.uk/id/eprint/142248
ISSN: 0379-864X
PURE UUID: e199a78e-f108-4b67-bb30-0c1ba3b1bf71
ORCID for Philip L. Newland: ORCID iD orcid.org/0000-0003-4124-8507

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Date deposited: 01 Apr 2010 14:31
Last modified: 14 Mar 2024 02:41

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Author: Paul Yates

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