The University of Southampton
University of Southampton Institutional Repository

The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for glucuronoxylan biosynthesis during secondary cell wall formation

Wu, Ai-Min, Rihouey, Christophe, Seveno, Marial, Hornblad, Emma, Singh, Sunil Kumar, Matsunaga, Toshiro, Ishii, Tadashi, Lerouge, Patrice and Marchant, Alan (2009) The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for glucuronoxylan biosynthesis during secondary cell wall formation The Plant Journal, 57, (4), pp. 718-731. (doi:10.1111/j.1365-313X.2008.03724.x). (PMID:18980649).

Record type: Article

Abstract

Arabidopsis IRX10 and IRX10-LIKE (IRX10-L) proteins are closely related members of the GT47 glycosyltransferase family. Single gene knock-outs of IRX10 or IRX10-L result in plants with either a weak or no mutant phenotype. However irx10 irx10-L double mutants are severely affected in their development, with a reduced rosette size and infrequent formation of a small infertile inflorescence. Plants homozygous for irx10 and heterozygous for irx10-L have an intermediate phenotype exhibiting a short inflorescence compared with the wild type, and an almost complete loss of fertility. Stem sections of the irx10 homozygous irx10-L heterozygous or irx10 irx10-L double mutants show decreased secondary cell-wall formation. NMR analysis shows that signals derived from the reducing end structure of glucuronoxylan were detected in the irx10 single mutant, and in the irx10 homozygous irx10-L heterozygous combination, but that the degree of polymerization of the xylan backbone was reduced compared with the wild type. Additionally, xylans from irx10 stem tissues have an almost complete loss of the GlcUA side chain, whereas the level of 4-O-Me-GlcUA was similar to that in wild type. Deletion of the predicted signal peptide from the N terminus of IRX10 or IRX10-L results in an inability to rescue the irx10 irx10-L double mutant phenotype. These findings demonstrate that IRX10 and IRX10-L perform a critical function in the synthesis of glucuronoxylan during secondary cell-wall formation, and that this activity is associated with the formation of the xylan backbone structure. This contrasts with the proposed function of the tobacco NpGUT1, which is closely related to the Arabidopsis IRX10 and IRX10-L proteins, in rhamnogalacturonan II biosynthesis.

Full text not available from this repository.

More information

e-pub ahead of print date: 27 November 2008
Published date: February 2009
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 142273
URI: http://eprints.soton.ac.uk/id/eprint/142273
ISSN: 0960-7412
PURE UUID: 8fab2fd3-9755-4056-9bcd-9b0c66fb0b77

Catalogue record

Date deposited: 01 Apr 2010 14:56
Last modified: 18 Jul 2017 23:12

Export record

Altmetrics

Contributors

Author: Ai-Min Wu
Author: Christophe Rihouey
Author: Marial Seveno
Author: Emma Hornblad
Author: Sunil Kumar Singh
Author: Toshiro Matsunaga
Author: Tadashi Ishii
Author: Patrice Lerouge
Author: Alan Marchant

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×