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Using image analysis to design a prototype autodissemination device intended for the biorational control of plodia interpunctella.

Using image analysis to design a prototype autodissemination device intended for the biorational control of plodia interpunctella.
Using image analysis to design a prototype autodissemination device intended for the biorational control of plodia interpunctella.
The extent to which a carrier powder was taken up and horizontally transferred by contaminated Indianmeal moth, Plodia interpunctella (Hübner) individuals to conspecifics was evaluated.

Using a marker dye, which was shown to be colocalized with the carrier powder, the amount of powder on the Indianmeal moth, as determined by spectrophotometry, was correlated with quantification based on a novel image analysis approach that enabled differentiation of powder uptake by body region.

Over a 48-h period, more powder was retained on the ventral surface than the dorsal side, with the head region showing the greatest amount of powder uptake and retention. During courtship, powder was horizontally transferred by treated males to 1.6% of the head and 0.06% of the untreated females' body.

To inoculate moths, a pit-fall style autodissemination station was determined as being more effective than a powder tray, as significantly more material was taken up to the key areas of the moth's body where powder is more effectively retained and more likely to be horizontally transferred.

Additionally, a pit-fall station prevented moths from exhibiting avoidance behavior from the powder, which was frequently encountered if they had to walk into a powder tray. This study shows that different regions of the Indianmeal moth body vary in capacity to carry powder and that future research efforts should target these specific regions.

Although the proposed autodissemination system was optimized for management strategies of Indianmeal moth, we believe the results presented here can be used to develop novel management strategies for other insect pests.
indianmeal moth, dissemination, powder, colocalization, imaging
453-465
Baxter, Ian H.
1d028199-1710-48a2-8851-6e8f713cd76e
Schuppe, Hansjurgen
16080883-aaa8-4d87-93e0-e1b4ff0bc71f
Jackson, Chris
ab14e7be-1b25-4425-9e8f-6ccee5b984a8
Nansen, Christian
4a776b14-dadd-4ada-8aef-6090770ca2cd
Baxter, Ian H.
1d028199-1710-48a2-8851-6e8f713cd76e
Schuppe, Hansjurgen
16080883-aaa8-4d87-93e0-e1b4ff0bc71f
Jackson, Chris
ab14e7be-1b25-4425-9e8f-6ccee5b984a8
Nansen, Christian
4a776b14-dadd-4ada-8aef-6090770ca2cd

Baxter, Ian H., Schuppe, Hansjurgen, Jackson, Chris and Nansen, Christian (2009) Using image analysis to design a prototype autodissemination device intended for the biorational control of plodia interpunctella. Journal of Economic Entomology, 102 (1), 453-465. (doi:10.1603/029.102.0159).

Record type: Article

Abstract

The extent to which a carrier powder was taken up and horizontally transferred by contaminated Indianmeal moth, Plodia interpunctella (Hübner) individuals to conspecifics was evaluated.

Using a marker dye, which was shown to be colocalized with the carrier powder, the amount of powder on the Indianmeal moth, as determined by spectrophotometry, was correlated with quantification based on a novel image analysis approach that enabled differentiation of powder uptake by body region.

Over a 48-h period, more powder was retained on the ventral surface than the dorsal side, with the head region showing the greatest amount of powder uptake and retention. During courtship, powder was horizontally transferred by treated males to 1.6% of the head and 0.06% of the untreated females' body.

To inoculate moths, a pit-fall style autodissemination station was determined as being more effective than a powder tray, as significantly more material was taken up to the key areas of the moth's body where powder is more effectively retained and more likely to be horizontally transferred.

Additionally, a pit-fall station prevented moths from exhibiting avoidance behavior from the powder, which was frequently encountered if they had to walk into a powder tray. This study shows that different regions of the Indianmeal moth body vary in capacity to carry powder and that future research efforts should target these specific regions.

Although the proposed autodissemination system was optimized for management strategies of Indianmeal moth, we believe the results presented here can be used to develop novel management strategies for other insect pests.

Full text not available from this repository.

More information

Published date: February 2009
Keywords: indianmeal moth, dissemination, powder, colocalization, imaging

Identifiers

Local EPrints ID: 142455
URI: https://eprints.soton.ac.uk/id/eprint/142455
PURE UUID: 639a770e-5144-42d3-8d6d-9f216a1fa8f6

Catalogue record

Date deposited: 01 Apr 2010 15:27
Last modified: 18 Jul 2017 23:11

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