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Maternal protein restriction with or without folic acid supplementation during pregnancy alters the hepatic transcriptome in adult male rats

Maternal protein restriction with or without folic acid supplementation during pregnancy alters the hepatic transcriptome in adult male rats
Maternal protein restriction with or without folic acid supplementation during pregnancy alters the hepatic transcriptome in adult male rats
Feeding pregnant rats a protein-restricted (PR) diet induces altered expression of candidate genes in the liver of the adult offspring, which can be prevented by supplementation of the PR diet with folic acid (PRF). We investigated the effect of maternal nutrition during pregnancy on the liver transcriptome in their adult male offspring. Pregnant rats were fed control, PR or PRF diets. Male offspring were killed on day 84. The liver transcriptome was analysed by microarray (six livers per maternal dietary group) followed by post hoc analysis of relative mRNA levels and gene ontology. These results were confirmed for selected genes by real-time RT-PCR. There were 311 genes that differed significantly ( >/= 1.5-fold change; P < 0.05) between PR offspring (222 increased) and control offspring, while 191 genes differed significantly between PRF offspring (forty-five increased) compared with offspring of control dams. There were sixteen genes that were significantly altered in both PR and PRF offspring compared with controls. Ion transport, developmental process, and response to reactive oxygen species (RROS) and steroid hormone response (SHR) ontologies were altered in PR offspring. Folic acid supplementation prevented changes within RROS and SHR response pathways, but not in ion transport or developmental process. There was no effect of maternal PR on mRNA expression of imprinted genes. Insulin 1 and Pleckstrin homology-like domain family A member 2 were increased significantly in PRF compared with PR offspring. The present findings show that the pattern of induced changes in the adult liver transcriptome were dependent on maternal protein and folic acid intakes during pregnancy.
transcriptome, maternal diet, microarray analysis, protein restriction, folate
0007-1145
1711-1719
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Rodford, Joanne
ebcf7754-85d4-4a6d-a0e7-f489fae363e7
Garratt, Emma S.
66ddd4cb-19a2-4d08-889b-12f418e6878b
Slater-Jefferies, Joanne L.
e46c711a-9d4c-436a-b853-828df69bb4d7
Godfrey, Keith M.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Gluckman, Peter D.
ef2e8b92-0b76-4a12-bd7c-01b0674f94d3
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Rodford, Joanne
ebcf7754-85d4-4a6d-a0e7-f489fae363e7
Garratt, Emma S.
66ddd4cb-19a2-4d08-889b-12f418e6878b
Slater-Jefferies, Joanne L.
e46c711a-9d4c-436a-b853-828df69bb4d7
Godfrey, Keith M.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Gluckman, Peter D.
ef2e8b92-0b76-4a12-bd7c-01b0674f94d3
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159

Lillycrop, K.A., Rodford, Joanne, Garratt, Emma S., Slater-Jefferies, Joanne L., Godfrey, Keith M., Gluckman, Peter D., Hanson, Mark A. and Burdge, Graham C. (2010) Maternal protein restriction with or without folic acid supplementation during pregnancy alters the hepatic transcriptome in adult male rats. British Journal of Nutrition, 103 (12), 1711-1719. (doi:10.1017/S0007114509993795). (PMID:20211039)

Record type: Article

Abstract

Feeding pregnant rats a protein-restricted (PR) diet induces altered expression of candidate genes in the liver of the adult offspring, which can be prevented by supplementation of the PR diet with folic acid (PRF). We investigated the effect of maternal nutrition during pregnancy on the liver transcriptome in their adult male offspring. Pregnant rats were fed control, PR or PRF diets. Male offspring were killed on day 84. The liver transcriptome was analysed by microarray (six livers per maternal dietary group) followed by post hoc analysis of relative mRNA levels and gene ontology. These results were confirmed for selected genes by real-time RT-PCR. There were 311 genes that differed significantly ( >/= 1.5-fold change; P < 0.05) between PR offspring (222 increased) and control offspring, while 191 genes differed significantly between PRF offspring (forty-five increased) compared with offspring of control dams. There were sixteen genes that were significantly altered in both PR and PRF offspring compared with controls. Ion transport, developmental process, and response to reactive oxygen species (RROS) and steroid hormone response (SHR) ontologies were altered in PR offspring. Folic acid supplementation prevented changes within RROS and SHR response pathways, but not in ion transport or developmental process. There was no effect of maternal PR on mRNA expression of imprinted genes. Insulin 1 and Pleckstrin homology-like domain family A member 2 were increased significantly in PRF compared with PR offspring. The present findings show that the pattern of induced changes in the adult liver transcriptome were dependent on maternal protein and folic acid intakes during pregnancy.

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More information

Published date: 2010
Keywords: transcriptome, maternal diet, microarray analysis, protein restriction, folate
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 143843
URI: http://eprints.soton.ac.uk/id/eprint/143843
ISSN: 0007-1145
PURE UUID: 5e89a46b-b2a3-4190-8e51-db5f006eeafc
ORCID for K.A. Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489
ORCID for Emma S. Garratt: ORCID iD orcid.org/0000-0001-5268-4203
ORCID for Joanne L. Slater-Jefferies: ORCID iD orcid.org/0000-0001-8325-1320
ORCID for Keith M. Godfrey: ORCID iD orcid.org/0000-0002-4643-0618
ORCID for Mark A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967

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Date deposited: 13 Apr 2010 12:13
Last modified: 14 Mar 2024 02:51

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Contributors

Author: K.A. Lillycrop ORCID iD
Author: Joanne Rodford
Author: Emma S. Garratt ORCID iD
Author: Peter D. Gluckman
Author: Mark A. Hanson ORCID iD

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