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Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes

Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes
Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes
Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the natural product prodigiosin, a tripyrrolic molecule produced by microorganisms such as Streptomyces and Serratia, facilitates chloride/bicarbonate exchange (antiport) across liposomal membranes. Higher concentrations of simple synthetic molecules based on a 4,6-dihydroxyisophthalamide core are also shown to facilitate this antiport process. Although it is well known that proteins regulate Cl-/HCO3- exchange in cells, these results suggest that small molecules may also be able to regulate the concentration of these anions in biological systems.
1755-4330
138-144
Davis, Jeffery T.
d1915d43-c934-43ec-afa8-0a0c4b309c4f
Gale, Philip A.
c840b7e9-6847-4843-91af-fa0f8563d943
Okunola, Oluyomi A.
f534cfff-c134-4ecd-aab8-1c349fb926d1
Prados, Pilar
2213bc76-d01c-44ee-b6c0-090c428a8eff
Iglesias-Sánchez, Jose Carlos
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Torroba, Tomás
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Quesada, Roberto
c7db6299-4eb5-490d-9e6b-be4b9fbc8ca9
Davis, Jeffery T.
d1915d43-c934-43ec-afa8-0a0c4b309c4f
Gale, Philip A.
c840b7e9-6847-4843-91af-fa0f8563d943
Okunola, Oluyomi A.
f534cfff-c134-4ecd-aab8-1c349fb926d1
Prados, Pilar
2213bc76-d01c-44ee-b6c0-090c428a8eff
Iglesias-Sánchez, Jose Carlos
3ab4ee6d-d90c-4232-856e-af2c764c0280
Torroba, Tomás
46c4486a-d3a4-493b-b972-112c546d3d7c
Quesada, Roberto
c7db6299-4eb5-490d-9e6b-be4b9fbc8ca9

Davis, Jeffery T., Gale, Philip A., Okunola, Oluyomi A., Prados, Pilar, Iglesias-Sánchez, Jose Carlos, Torroba, Tomás and Quesada, Roberto (2009) Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes. Nature Chemistry, 1 (2), 138-144. (doi:10.1038/nchem.178).

Record type: Article

Abstract

Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the natural product prodigiosin, a tripyrrolic molecule produced by microorganisms such as Streptomyces and Serratia, facilitates chloride/bicarbonate exchange (antiport) across liposomal membranes. Higher concentrations of simple synthetic molecules based on a 4,6-dihydroxyisophthalamide core are also shown to facilitate this antiport process. Although it is well known that proteins regulate Cl-/HCO3- exchange in cells, these results suggest that small molecules may also be able to regulate the concentration of these anions in biological systems.

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Published date: May 2009
Organisations: Chemistry

Identifiers

Local EPrints ID: 144923
URI: http://eprints.soton.ac.uk/id/eprint/144923
ISSN: 1755-4330
PURE UUID: 5f1b7cfc-f01a-4646-aea7-9975cd8a9c02
ORCID for Philip A. Gale: ORCID iD orcid.org/0000-0001-9751-4910

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Date deposited: 15 Apr 2010 13:08
Last modified: 03 Dec 2019 01:54

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Contributors

Author: Jeffery T. Davis
Author: Philip A. Gale ORCID iD
Author: Oluyomi A. Okunola
Author: Pilar Prados
Author: Jose Carlos Iglesias-Sánchez
Author: Tomás Torroba
Author: Roberto Quesada

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