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Expression of the small heat shock protein family in the mouse CNS: differential anatomical and biochemical compartmentalization

Quraishe, S., Asuni, A., Boelens, W.C., O'Connor, V. and Wyttenbach, A. (2008) Expression of the small heat shock protein family in the mouse CNS: differential anatomical and biochemical compartmentalization Neuroscience, 153, (2), pp. 483-491. (doi:10.1016/j.neuroscience.2008.01.058).

Record type: Article

Abstract

The small heat shock proteins (sHsps) are a family of molecular chaperones defined by an alpha-crystallin domain that is important for sHsps oligomerization and chaperone activity. sHsps perform many physiological functions including the maintenance of the cellular cytoskeleton, the regulation of protein aggregation and modulate cell survival in a number of cell types including glial and neuronal cells.

Many of these functions have been implicated in disease processes in the CNS and indeed sHsps are considered targets for disease therapy. Despite this, there is no study that systematically and comparatively characterized sHsps expression in the CNS. In the present study we have analyzed the expression of this gene family in the mouse brain by reverse-transcriptase polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting. Gene expression analysis of the 10 known members of mammalian sHsps confirms the presence of 5 sHsps in the CNS.

A distinct white matter specific expression pattern for HspB5 and overlapping expression of HspB1 and HspB8 in the lateral and dorsal ventricles of the brain is observed. We confirm protein expression of HspB1, HspB5, HspB6 and HspB8 in the brain. Further subcellular fractionation of brain and synaptosomes details a distinct subcompartment-specific association and detergent solubility of sHsps.

This biochemical signature is indicative of an association with synaptic and other neural specializations. This observation will help one understand the functional role played by sHsps during physiology and pathology in the CNS.

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More information

Published date: 2 May 2008
Keywords: small heat shock proteins, solubility, brain fractionation, synapse
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 145329
URI: http://eprints.soton.ac.uk/id/eprint/145329
ISSN: 0306-4522
PURE UUID: d88c67e6-2a45-4774-8794-900ff6bdde9c

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Date deposited: 19 Apr 2010 08:46
Last modified: 18 Jul 2017 23:06

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Contributors

Author: S. Quraishe
Author: A. Asuni
Author: W.C. Boelens
Author: V. O'Connor
Author: A. Wyttenbach

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