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Polymorphisms in the interleukin 13 and GATA binding protein 3 genes and the development of eczema during childhood

Polymorphisms in the interleukin 13 and GATA binding protein 3 genes and the development of eczema during childhood
Polymorphisms in the interleukin 13 and GATA binding protein 3 genes and the development of eczema during childhood
Background: atopic eczema is characterized by Th2-dominant immunity with the cytokine interleukin 13 and the transcription factor GATA binding protein 3 playing a critical role.

Objectives: we assessed the association of polymorphisms in the IL13 and GATA3 genes with childhood eczema.

Methods: a birth cohort (n = 1456) was established on the Isle of Wight in 1989 and followed at the ages of 1 (n = 1167), 2 (n = 1174), 4 (n = 1218) and 10 years (n = 1373) to determine the prevalence of allergic disease including eczema. At 4 and 10 years, skin prick testing was performed. Whole blood samples (n = 923) were obtained at the 10-year assessment, stored frozen, and genotyped. Five polymorphisms from IL13 and seven from GATA3 were genotyped for this analysis. Repeated measurement analyses were conducted for the occurrence of eczema at ages 1, 2, 4 and 10 years. All analyses were adjusted for maternal and paternal eczema, low birth weight (< 2500 g), breastfeeding >or= 3 months and age.

Results: IL13 was not associated with childhood eczema. For GATA3, the single nucleotide polymorphism (SNP) rs2275806 (promoter region) showed an increased odds ratio for atopic eczema independent of whether the comparison group had a positive skin prick test. The SNP rs444762 (intron 3 region) was associated with atopic eczema in comparison with children without eczema. The increased relative risks remained significant after adjustment for multiple testing only for rs2275806 (P < 0.05).

Conclusions: a SNP in GATA3 is associated with atopic eczema. This finding highlights the importance of GATA3 as an immune-modulating gene in atopic eczema.
atopy, cytokines, eczema, genetics, single nucleotide polymorphisms
0007-0963
1315-1322
Arshad, S.H.
917e246d-2e60-472f-8d30-94b01ef28958
Karmaus, W.
d78616d6-bc9c-4664-a461-7c0d0be5e39e
Kurukulaaratchy, R.
9c7b8105-2892-49f2-8775-54d4961e3e74
Sadeghnejad, A.
254a27f3-f46c-41da-a9a5-f1c1651fb4ff
Huebner, M.
5d64fdac-cb4a-4acd-a8b2-cd530f66e503
Ewart, S.
9da730b1-7e8a-4889-8dc5-2703de288a34
Arshad, S.H.
917e246d-2e60-472f-8d30-94b01ef28958
Karmaus, W.
d78616d6-bc9c-4664-a461-7c0d0be5e39e
Kurukulaaratchy, R.
9c7b8105-2892-49f2-8775-54d4961e3e74
Sadeghnejad, A.
254a27f3-f46c-41da-a9a5-f1c1651fb4ff
Huebner, M.
5d64fdac-cb4a-4acd-a8b2-cd530f66e503
Ewart, S.
9da730b1-7e8a-4889-8dc5-2703de288a34

Arshad, S.H., Karmaus, W., Kurukulaaratchy, R., Sadeghnejad, A., Huebner, M. and Ewart, S. (2008) Polymorphisms in the interleukin 13 and GATA binding protein 3 genes and the development of eczema during childhood. British Journal of Dermatology, 158 (6), 1315-1322. (doi:10.1111/j.1365-2133.2008.08565.x).

Record type: Article

Abstract

Background: atopic eczema is characterized by Th2-dominant immunity with the cytokine interleukin 13 and the transcription factor GATA binding protein 3 playing a critical role.

Objectives: we assessed the association of polymorphisms in the IL13 and GATA3 genes with childhood eczema.

Methods: a birth cohort (n = 1456) was established on the Isle of Wight in 1989 and followed at the ages of 1 (n = 1167), 2 (n = 1174), 4 (n = 1218) and 10 years (n = 1373) to determine the prevalence of allergic disease including eczema. At 4 and 10 years, skin prick testing was performed. Whole blood samples (n = 923) were obtained at the 10-year assessment, stored frozen, and genotyped. Five polymorphisms from IL13 and seven from GATA3 were genotyped for this analysis. Repeated measurement analyses were conducted for the occurrence of eczema at ages 1, 2, 4 and 10 years. All analyses were adjusted for maternal and paternal eczema, low birth weight (< 2500 g), breastfeeding >or= 3 months and age.

Results: IL13 was not associated with childhood eczema. For GATA3, the single nucleotide polymorphism (SNP) rs2275806 (promoter region) showed an increased odds ratio for atopic eczema independent of whether the comparison group had a positive skin prick test. The SNP rs444762 (intron 3 region) was associated with atopic eczema in comparison with children without eczema. The increased relative risks remained significant after adjustment for multiple testing only for rs2275806 (P < 0.05).

Conclusions: a SNP in GATA3 is associated with atopic eczema. This finding highlights the importance of GATA3 as an immune-modulating gene in atopic eczema.

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More information

Published date: June 2008
Keywords: atopy, cytokines, eczema, genetics, single nucleotide polymorphisms

Identifiers

Local EPrints ID: 145779
URI: https://eprints.soton.ac.uk/id/eprint/145779
ISSN: 0007-0963
PURE UUID: e130e4e9-0d77-4380-98d9-35d01a38fa91

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Date deposited: 19 Apr 2010 14:45
Last modified: 17 Jul 2019 00:06

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